Article: article from journal or magazin.
Three-dimensional immunogold labeling of nuclear matrix proteins in permeabilized cells.
Cell Biology International Reports
A preembedment labeling procedure is described for the three-dimensional (3D) labeling of nuclear matrix proteins in permeabilized cells. The procedure is based on the use of ultra-small (1 nm) gold particles as a marker system. This marker penetrates the nucleus more efficiently than the conventionally used 5-10 nm colloidal gold probes. Dehydration is performed by freeze-substitution to preserve the ultrastructure of the cell as optimally as possible. During freeze-substitution the samples are stained by uranyl ions to stain the cellular material throughout the resin section. The 3D gold-labeled and uranyl-stained specimen is embedded in Epon resin and semi-thin (0.2-0.5 microns) sections are made for stereo electron microscopy. The applicability of this method is illustrated by the localization of nuclear matrix-associated nuclear bodies in permeabilized interphase and mitotic HeLa cells.
Cell Membrane Permeability/drug effects, Epoxy Resins, Freeze Substitution, Gold, HeLa Cells/ultrastructure, Humans, Immunohistochemistry, Microscopy, Immunoelectron/methods, Nuclear Matrix/chemistry, Nuclear Matrix/ultrastructure, Nuclear Proteins/analysis, Octoxynol, Polyethylene Glycols/pharmacology
Web of science
Last modification date