Copper Staining/Labeling and Scanning Electrochemical Microscopy Readout of Proteins on Poly(vinylidene difluoride) Membranes

Details

Serval ID
serval:BIB_3943ED1FCF6C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Copper Staining/Labeling and Scanning Electrochemical Microscopy Readout of Proteins on Poly(vinylidene difluoride) Membranes
Journal
CHIMIA International Journal for Chemistry
Author(s)
Carano Maurizio, Lion Niels, Girault Hubert H.
ISSN
0009-4293 (Print)
ISSN-L
0000-0000 (Electronic)
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
59
Number
3
Pages
105-108
Language
english
Abstract
Scanning Electrochemical Microscopy (SECM) is used in combination with copper labeling to visualize proteins on surfaces. Proteins are adsorbed on a poly(vinylidene difiuoride) (PVDF) membrane and stained using a standard protocol involving copper salts. The latter are then reduced to copper metal and further detected by SECM with ferrocene methanol as a redox mediator in aqueous solution. During the SECM scan, the potential is held at a value at which the oxidation of the redox mediator occurs and a, positive feedback current is detected when scanning over copper clusters. A negative feedback is observed elsewhere.
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Create date
09/11/2014 23:30
Last modification date
20/08/2019 13:28
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