(MA)LDI MS Imaging at High Specificity and Sensitivity

Détails

ID Serval
serval:BIB_391B5365B47F
Type
Partie de livre
Sous-type
Chapitre: chapitre ou section
Collection
Publications
Titre
(MA)LDI MS Imaging at High Specificity and Sensitivity
Titre du livre
Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry
Auteur(s)
Thomas A., Heath Patterson N., Dufresne M., Chaurand P.
Editeur
Springer
ISBN
9783319048185
Statut éditorial
Publié
Date de publication
2016
Pages
129-147
Langue
anglais
Résumé
(Matrix-assisted) laser desorption/ionization ((MA)LDI) mass spectrometry imaging (MSI) has been driven by remarkable technological developments in the last couple of years. Although molecular information of a wide range of molecules including peptides, lipids, metabolites, and xenobiotics can be mapped, (MA)LDI MSI only leads to the detection of the most abundant soluble molecules in the cells and, consequently, does not provide access to the least expressed species, which can be very informative in the scope of disease research. Within a short period of time, numerous protocols and concepts have been developed and introduced in order to increase MSI sensitivity, including in situ tissue chemistry and solvent-free matrix depositions. In this chapter, we will discuss some of the latest developments in the field of high-sensitivity MSI using solvent-free matrix depositions and will detail protocols of two methods with their capability of enriching molecular MSI signal as demonstrated within our laboratory.
Mots-clé
MALDI Mass spectrometry Imaging Tissue 1,5-diaminonaphtalene Sublimation Silver Sputtering Specificity Sensitivity Lipids Fatty acids Cholesterol
Création de la notice
05/01/2016 9:57
Dernière modification de la notice
03/03/2018 16:12
Données d'usage