PHO1 expression in guard cells mediates the stomatal response to abscisic acid in Arabidopsis.

Détails

Ressource 1Télécharger: BIB_391516688BBD.P001.pdf (731.18 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_391516688BBD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
PHO1 expression in guard cells mediates the stomatal response to abscisic acid in Arabidopsis.
Périodique
Plant Journal
Auteur(s)
Zimmerli C., Ribot C., Vavasseur A., Bauer H., Hedrich R., Poirier Y.
ISSN
1365-313X (Electronic)
ISSN-L
0960-7412
Statut éditorial
Publié
Date de publication
2012
Peer-reviewed
Oui
Volume
72
Numéro
2
Pages
199-211
Langue
anglais
Résumé
Stomatal opening and closing are driven by ion fluxes that cause changes in guard cell turgor and volume. This process is, in turn, regulated by environmental and hormonal signals, including light and the phytohormone abscisic acid (ABA). Here, we present genetic evidence that expression of PHO1 in guard cells of Arabidopsis thaliana is required for full stomatal responses to ABA. PHO1 is involved in the export of phosphate into the root xylem vessels and, as a result, the pho1 mutant is characterized by low shoot phosphate levels. In leaves, PHO1 was found expressed in guard cells and up-regulated following treatment with ABA. The pho1 mutant was unaffected in production of reactive oxygen species following ABA treatment, and in stomatal movements in response to light cues, high extracellular calcium, auxin, and fusicoccin. However, stomatal movements in response to ABA treatment were severely impaired, both in terms of induction of closure and inhibition of opening. Micro-grafting a pho1 shoot scion onto wild-type rootstock resulted in plants with normal shoot growth and phosphate content, but failed to restore normal stomatal response to ABA treatment. PHO1 knockdown using RNA interference specifically in guard cells of wild-type plants caused a reduced stomatal response to ABA. In agreement, specific expression of PHO1 in guard cells of pho1 plants complemented the mutant guard cell phenotype and re-established ABA sensitivity, although full functional complementation was dependent on shoot phosphate sufficiency. Together, these data reveal an important role for phosphate and the action of PHO1 in the stomatal response to ABA.
Mots-clé
Abscisic Acid/pharmacology, Arabidopsis/drug effects, Arabidopsis/genetics, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Biological Transport, Gene Expression Regulation, Plant, Gene Knockdown Techniques, Genetic Complementation Test, Light, Mutation, Organ Specificity, Phenotype, Phosphates/analysis, Phosphates/metabolism, Plant Epidermis/drug effects, Plant Epidermis/genetics, Plant Growth Regulators/pharmacology, Plant Leaves/drug effects, Plant Leaves/genetics, Plant Roots/drug effects, Plant Roots/genetics, Plant Stomata/drug effects, Plant Stomata/genetics, Reactive Oxygen Species/metabolism, Signal Transduction/drug effects, Up-Regulation, Xylem/drug effects, Xylem/genetics
Pubmed
Web of science
Création de la notice
19/12/2012 10:10
Dernière modification de la notice
03/03/2018 16:12
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