Disentangling metabolic functions of bacteria in the honey bee gut.

Détails

Ressource 1Télécharger: journal.pbio.2003467.pdf (3200.44 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_3880396A281C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Disentangling metabolic functions of bacteria in the honey bee gut.
Périodique
PLoS biology
Auteur(s)
Kešnerová L., Mars RAT, Ellegaard K.M., Troilo M., Sauer U., Engel P.
ISSN
1545-7885 (Electronic)
ISSN-L
1544-9173
Statut éditorial
Publié
Date de publication
12/2017
Peer-reviewed
Oui
Volume
15
Numéro
12
Pages
e2003467
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
It is presently unclear how much individual community members contribute to the overall metabolic output of a gut microbiota. To address this question, we used the honey bee, which harbors a relatively simple and remarkably conserved gut microbiota with striking parallels to the mammalian system and importance for bee health. Using untargeted metabolomics, we profiled metabolic changes in gnotobiotic bees that were colonized with the complete microbiota reconstituted from cultured strains. We then determined the contribution of individual community members in mono-colonized bees and recapitulated our findings using in vitro cultures. Our results show that the honey bee gut microbiota utilizes a wide range of pollen-derived substrates, including flavonoids and outer pollen wall components, suggesting a key role for degradation of recalcitrant secondary plant metabolites and pollen digestion. In turn, multiple species were responsible for the accumulation of organic acids and aromatic compound degradation intermediates. Moreover, a specific gut symbiont, Bifidobacterium asteroides, stimulated the production of host hormones known to impact bee development. While we found evidence for cross-feeding interactions, approximately 80% of the identified metabolic changes were also observed in mono-colonized bees, with Lactobacilli being responsible for the largest share of the metabolic output. These results show that, despite prolonged evolutionary associations, honey bee gut bacteria can independently establish and metabolize a wide range of compounds in the gut. Our study reveals diverse bacterial functions that are likely to contribute to bee health and provide fundamental insights into how metabolic activities are partitioned within gut communities.

Mots-clé
Animals, Bacteria/isolation & purification, Bacteria/metabolism, Bees/metabolism, Bees/microbiology, Fermentation, Flavonoids/metabolism, Food Chain, Gastrointestinal Microbiome/physiology, Metabolomics, Nucleosides/metabolism, Pollen/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
14/12/2017 18:22
Dernière modification de la notice
08/05/2019 17:09
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