Urokinase receptor (uPAR, CD87) is a platelet receptor important for kinetics and TNF-induced endothelial adhesion in mice.

Détails

ID Serval
serval:BIB_385860297736
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Urokinase receptor (uPAR, CD87) is a platelet receptor important for kinetics and TNF-induced endothelial adhesion in mice.
Périodique
Circulation
Auteur(s)
Piguet P.F., Vesin C., Donati Y., Tacchini-Cottier F., Belin D., Barazzone C.
ISSN
1524-4539 (Electronic)
ISSN-L
0009-7322
Statut éditorial
Publié
Date de publication
1999
Volume
99
Numéro
25
Pages
3315-3321
Langue
anglais
Résumé
BACKGROUND: Urokinase plasminogen activator receptor (uPAR, CD87) is a widely distributed 55-kD, glycoprotein I-anchored surface receptor. On binding of its ligand uPA, it is known to increase leukocyte adhesion and traffic. Using genetically deficient mice, we explored the role of uPAR in platelet kinetics and TNF-induced platelet consumption.
METHODS AND RESULTS: Anti-uPAR antibody stained platelets from normal (+/+) but not from uPAR-/- mice, as seen by fluorescence-activated cell sorter analysis. 51Cr-labeled platelets from uPAR-/- donors survived longer than those from +/+ donors when injected into a +/+ recipient. Intratracheal TNF injection induced thrombocytopenia and a platelet pulmonary localization, pronounced in +/+ but absent in uPAR-/- mice. Aprotinin, a plasmin inhibitor, decreased TNF-induced thrombocytopenia. TNF injection markedly reduced the survival and increased the pulmonary localization of 51Cr-labeled platelets from +/+ but not from uPAR-/- donors, indicating that it is the platelet uPAR that is critical for their response to TNF. As seen by electron microscopy, TNF injection increased the number of platelets and polymorphonuclear neutrophils (PMNs) in the alveolar capillaries of +/+ mice, whereas in uPAR-/- mice, platelet trapping was insignificant and PMN trapping was slightly reduced. Platelets within alveolar capillaries of TNF-injected mice were activated, as judged from their shape, and this was evident in +/+ but not in uPAR-/- mice.
CONCLUSIONS: These results demonstrate for the first time the critical role of platelet uPAR for kinetics as well as for activation and endothelium adhesion associated with inflammation.
Mots-clé
Animals, Aprotinin/pharmacology, Capillaries, Cell Adhesion, Cell Survival, Chromium Radioisotopes/diagnostic use, Endothelium, Vascular/cytology, Endothelium, Vascular/metabolism, Flow Cytometry, Injections, Kinetics, Mice, Mice, Inbred C57BL, Neutrophils/metabolism, Plasminogen Activators/metabolism, Plasminogen Activators/pharmacology, Pulmonary Alveoli/blood supply, Receptors, Cell Surface/metabolism, Receptors, Urokinase Plasminogen Activator, Recombinant Proteins/metabolism, Recombinant Proteins/pharmacology, Thrombocytopenia/metabolism, Thrombocytopenia/prevention & control, Tumor Necrosis Factor-alpha/antagonists & inhibitors, Tumor Necrosis Factor-alpha/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 16:09
Dernière modification de la notice
20/08/2019 14:27
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