Low Mannan-binding lectin serum levels are associated with complicated Crohn's disease and reactivity to oligomannan (ASCA).

Details

Serval ID
serval:BIB_37D2CDED9655
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Low Mannan-binding lectin serum levels are associated with complicated Crohn's disease and reactivity to oligomannan (ASCA).
Journal
The American journal of gastroenterology
Author(s)
Schoepfer Alain M., Flogerzi Beatrice, Seibold-Schmid Beatrice, Schaffer Thomas, Kun Jürgen F.J., Pittet Valérie, Mueller Stefan, Seibold Frank
Working group(s)
Swiss Inflammatory Bowel Disease Cohort Study (SIBDCS)
ISSN
1572-0241[electronic]
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
104
Number
10
Pages
2508-2516
Language
english
Abstract
OBJECTIVES: Mannan-binding lectin (MBL) acts as a pattern-recognition molecule directed against oligomannan, which is part of the cell wall of yeasts and various bacteria. We have previously shown an association between MBL deficiency and anti-Saccharomyces cerevisiae mannan antibody (ASCA) positivity. This study aims at evaluating whether MBL deficiency is associated with distinct Crohn's disease (CD) phenotypes. METHODS: Serum concentrations of MBL and ASCA were measured using ELISA (enzyme-linked immunosorbent assay) in 427 patients with CD, 70 with ulcerative colitis, and 76 healthy controls. CD phenotypes were grouped according to the Montreal Classification as follows: non-stricturing, non-penetrating (B1, n=182), stricturing (B2, n=113), penetrating (B3, n=67), and perianal disease (p, n=65). MBL was classified as deficient (<100 ng/ml), low (100-500 ng/ml), and normal (500 ng/ml). RESULTS: Mean MBL was lower in B2 and B3 CD patients (1,503+/-1,358 ng/ml) compared with that in B1 phenotypes (1,909+/-1,392 ng/ml, P=0.013). B2 and B3 patients more frequently had low or deficient MBL and ASCA positivity compared with B1 patients (P=0.004 and P<0.001). Mean MBL was lower in ASCA-positive CD patients (1,562+/-1,319 ng/ml) compared with that in ASCA-negative CD patients (1,871+/-1,320 ng/ml, P=0.038). In multivariate logistic regression modeling, low or deficient MBL was associated significantly with B1 (negative association), complicated disease (B2+B3), and ASCA. MBL levels did not correlate with disease duration. CONCLUSIONS: Low or deficient MBL serum levels are significantly associated with complicated (stricturing and penetrating) CD phenotypes but are negatively associated with the non-stricturing, non-penetrating group. Furthermore, CD patients with low or deficient MBL are significantly more often ASCA positive, possibly reflecting delayed clearance of oligomannan-containing microorganisms by the innate immune system in the absence of MBL.
Keywords
Adolescent, Adult, Aged, Aged, 80 and over, Antibodies, Fungal/blood, Case-Control Studies, Chi-Square Distribution, Crohn Disease/blood, Crohn Disease/complications, Enzyme-Linked Immunosorbent Assay, Female, Humans, Logistic Models, Male, Mannans/blood, Mannose-Binding Lectin/blood, Mannose-Binding Lectin/deficiency, Middle Aged, Phenotype, Polymerase Chain Reaction, Saccharomyces cerevisiae/immunology, Switzerland
Pubmed
Web of science
Create date
06/08/2009 14:30
Last modification date
20/08/2019 13:26
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