SosA inhibits cell division in Staphylococcus aureus in response to DNA damage.

Détails

ID Serval
serval:BIB_37C40301ACDA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
SosA inhibits cell division in Staphylococcus aureus in response to DNA damage.
Périodique
Molecular microbiology
Auteur(s)
Bojer M.S., Wacnik K., Kjelgaard P., Gallay C., Bottomley A.L., Cohn M.T., Lindahl G., Frees D., Veening J.W., Foster S.J., Ingmer H.
ISSN
1365-2958 (Electronic)
ISSN-L
0950-382X
Statut éditorial
Publié
Date de publication
10/2019
Peer-reviewed
Oui
Volume
112
Numéro
4
Pages
1116-1130
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Inhibition of cell division is critical for viability under DNA-damaging conditions. DNA damage induces the SOS response that in bacteria inhibits cell division while repairs are being made. In coccoids, such as the human pathogen, Staphylococcus aureus, this process remains poorly studied. Here, we identify SosA as the staphylococcal SOS-induced cell division inhibitor. Overproduction of SosA inhibits cell division, while sosA inactivation sensitizes cells to genotoxic stress. SosA is a small, predicted membrane protein with an extracellular C-terminal domain in which point mutation of residues that are conserved in staphylococci and major truncations abolished the inhibitory activity. In contrast, a minor truncation led to SosA accumulation and a strong cell division inhibitory activity, phenotypically similar to expression of wild-type SosA in a CtpA membrane protease mutant. This suggests that the extracellular C-terminus of SosA is required both for cell division inhibition and for turnover of the protein. Microscopy analysis revealed that SosA halts cell division and synchronizes the cell population at a point where division proteins such as FtsZ and EzrA are localized at midcell, and the septum formation is initiated but unable to progress to closure. Thus, our findings show that SosA is central in cell division regulation in staphylococci.
Mots-clé
S. aureus, CtpA, SOS response, SosA, cell division, cell-division inhibition
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/07/2019 14:47
Dernière modification de la notice
23/10/2019 5:13
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