Structure of helical RecA-DNA complexes. Complexes formed in the presence of ATP-gamma-S or ATP.

Détails

ID Serval
serval:BIB_3783C44930F1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Structure of helical RecA-DNA complexes. Complexes formed in the presence of ATP-gamma-S or ATP.
Périodique
Journal of Molecular Biology
Auteur(s)
Egelman E.H., Stasiak A.
ISSN
0022-2836[print], 0022-2836[linking]
Statut éditorial
Publié
Date de publication
10/1986
Volume
191
Numéro
4
Pages
677-697
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
Electron micrographs of RecA-DNA filaments, formed under several different conditions, have been analyzed and the filament images reconstructed in three dimensions. In the presence of ATP and a non-hydrolyzable ATP analog. ATP-gamma-S, the RecA protein forms with DNA a right-handed helical complex with a pitch of approximately 95 A. The most detailed view of the filament was obtained from analysis of RecA filaments on double-stranded DNA in the presence of ATP-gamma-S. There are approximately six subunits of RecA per turn of the helix, but both this number and the pitch are variable. From the examination of single filaments and filament-filament interactions, a picture of an extremely flexible protein structure emerges. The subunits of RecA protein are seen to be arranged in such a manner that the bound DNA must be partially exposed and able to come into contact with external DNA molecules. The RecA structure determined in the presence of ATP-gamma-S appears to be the same as the "pre-synaptic" state that occurs with ATP, in which there is recognition and pairing between homologous DNA molecules.
Mots-clé
Adenosine Triphosphate/analogs &amp, derivatives, Bacteriophage phi X 174, Computer Simulation, DNA, Circular, DNA, Viral, Macromolecular Substances, Microscopy, Electron, Models, Biological, Rec A Recombinases, X-Ray Diffraction
Pubmed
Web of science
Création de la notice
24/01/2008 11:36
Dernière modification de la notice
03/03/2018 16:05
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