Analysis of interaction of cloned human and/or sheep fetal hemoglobin gamma-chain and LPS in augmenting induction of inflammatory cytokine production in vivo and in vitro.

Détails

ID Serval
serval:BIB_37776
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Analysis of interaction of cloned human and/or sheep fetal hemoglobin gamma-chain and LPS in augmenting induction of inflammatory cytokine production in vivo and in vitro.
Périodique
Immunology Letters
Auteur(s)
Gorczynski R.M., Alexander C., Bessler W., Fournier K., Hoffmann P., Mach J.P., Rietschel E.T., Song L., Waelli T., Westphal O., Zahringer U., Khatri I.
ISSN
0165-2478 (Print)
ISSN-L
0165-2478
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
100
Numéro
2
Pages
120-129
Langue
anglais
Résumé
We have reported earlier that purified preparations of sheep fetal hemoglobin, but not adult hemoglobin, in concert with non-stimulatory doses of lipopolysaccharide (LPS) (lipid A), act cooperatively to regulate in vitro production of a number of cytokines, including TNFalpha, TGFbeta and IL-6 from murine and human leukocytes. Following in vivo treatment of mice with the same combination of hemoglobin and LPS, harvested spleen or peritoneal cells showed a similar augmented capacity to release these cytokines into culture supernatants. We report below that genetically cloned gamma-chain of human or sheep fetal hemoglobin, but not cloned alpha- or beta-chains, can produce this cooperative effect, as indeed can HPLC purified, heme-free, gamma-chains derived from cord blood fetal hemoglobin, and that purified haptoglobin completely abolishes the cooperative interaction.
Mots-clé
Age Factors, Amino Acid Sequence, Animals, Cloning, Molecular, Cricetinae, Dose-Response Relationship, Drug, Fetal Hemoglobin/biosynthesis, Fetal Hemoglobin/genetics, Globins/biosynthesis, Globins/chemistry, Haptoglobins/pharmacology, Humans, Interleukin-6/biosynthesis, Lipid A/administration & dosage, Lipid A/antagonists & inhibitors, Lipopolysaccharides/administration & dosage, Lipopolysaccharides/immunology, Lymphocytes/drug effects, Mice, Molecular Sequence Data, Sheep, Spleen/cytology, Spleen/drug effects, Transforming Growth Factor beta/biosynthesis, Tumor Necrosis Factor-alpha/biosynthesis
Pubmed
Web of science
Création de la notice
19/11/2007 13:36
Dernière modification de la notice
03/03/2018 16:05
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