Reduced L-selectin (CD62LLow) expression identifies tumor-specific type 1 T cells from lymph nodes draining an autologous tumor cell vaccine.

Détails

ID Serval
serval:BIB_3771552B28C2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Reduced L-selectin (CD62LLow) expression identifies tumor-specific type 1 T cells from lymph nodes draining an autologous tumor cell vaccine.
Périodique
Cellular Immunology
Auteur(s)
Meijer S.L., Dols A., Hu H.M., Chu Y., Romero P., Urba W.J., Fox B.A.
ISSN
0008-8749
Statut éditorial
Publié
Date de publication
2004
Peer-reviewed
Oui
Volume
227
Numéro
2
Pages
93-102
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
Reduced expression of CD62L can identify tumor-specific T cells in lymph nodes draining murine tumors. Here, we examined whether this strategy could isolate tumor-specific T cells from vaccinated patients. Tumor vaccine-draining lymph node (TVDLN) T cells of seven patients were separated into populations with reduced (CD62LLow) or high levels of CD62L (CD62LHigh). Effector T cells generated from CD62LLow cells maintained or enriched the autologous tumor-specific type 1 cytokine response compared to unseparated TVDLN T cells in four of four patients showing tumor-specific cytokine secretion. Interestingly, effector T cells generated from CD62LLow or CD62LHigh TVDLN were polarized towards a dominant type 1 or type 2 cytokine profile, respectively. For CD62LLow T cells the type 1 cytokine profile appeared determined prior to culture. Since a tumor-specific type 1 cytokine profile appears critical for mediating anti-tumor activity in vivo, this approach might be used to isolate T cells for adoptive immunotherapy.
Mots-clé
Cancer Vaccines/immunology, Carcinoma, Renal Cell/immunology, Carcinoma, Renal Cell/therapy, Humans, Immunotherapy, Adoptive, Interferon-gamma/biosynthesis, Interleukin-5/biosynthesis, Kidney Neoplasms/immunology, Kidney Neoplasms/therapy, L-Selectin/analysis, Lymph Nodes/immunology, Melanoma/immunology, Melanoma/therapy, T-Lymphocytes/immunology
Pubmed
Web of science
Création de la notice
28/01/2008 12:28
Dernière modification de la notice
03/03/2018 16:05
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