Article: article from journal or magazin.
IL-4Ralpha signaling is important for CD8+ T cell cytotoxicity in the absence of CD4+ T cell help.
European Journal of Immunology
Cytotoxic CD8(+) T cells are key mediators of viral clearance during primary infection through their production of IFN-gamma and lysis of virally infected cells. Comparatively, the cytokines IL-4 and IL-13 are typically associated with the development of Th2 immune responses against allergens and parasites, while their influence on cytotoxic CD8(+) T cell responses is controversial. We have investigated the roles of IL-4 and IL-13 in the development of CD8(+) T cell responses against influenza infection. We show that in the absence of either IL-4 or IL-13, CD8(+) T cells proliferated and a normal secondary cytotoxic response developed in vitro. In striking contrast, the absence of IL-4Ralpha resulted in impaired ex vivo proliferation and consequently no secondary CTL activity, whereas the in vivo response appeared normal. We show that the presence of CD4(+) T cell help, or the addition of exogenous IL-2 in vitro, restored the response. Taken together, this work reveals previously unrecognized in vivo redundancies between IL-4, IL-13 and IL-2 during immune responses against influenza virus.
Animals, CD4-Positive T-Lymphocytes/immunology, CD8-Positive T-Lymphocytes/immunology, Cytotoxicity Tests, Immunologic, DNA-Binding Proteins/deficiency, DNA-Binding Proteins/immunology, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Interleukin-13/immunology, Interleukin-2/immunology, Interleukin-4/immunology, Lymphocyte Activation/immunology, Mice, Orthomyxoviridae Infections/immunology, Receptors, Cell Surface, Trans-Activators/deficiency, Trans-Activators/immunology
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