LC-MS/MS analysis and comparison of oxidative damages on peptides induced by pathogen reduction technologies for platelets.

Détails

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Etat: Serval
Version: Final published version
ID Serval
serval:BIB_373E7BA7910C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
LC-MS/MS analysis and comparison of oxidative damages on peptides induced by pathogen reduction technologies for platelets.
Périodique
Journal of the American Society For Mass Spectrometry
Auteur(s)
Prudent M., Sonego G., Abonnenc M., Tissot J.D., Lion N.
ISSN
1879-1123 (Electronic)
ISSN-L
1044-0305
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
25
Numéro
4
Pages
651-661
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
Pathogen reduction technologies (PRT) are photochemical processes that use a combination of photosensitizers and UV-light to inactivate pathogens in platelet concentrates (PCs), a blood-derived product used to prevent hemorrhage. However, different studies have questioned the impact of PRT on platelet function and transfusion efficacy, and several proteomic analyses revealed possible oxidative damages to proteins. The present work focused on the oxidative damages produced by the two main PRT on peptides. Model peptides containing residues prone to oxidation (tyrosine, histidine, tryptophane, and cysteine) were irradiated with a combination of amotosalen/UVA (Intercept process) or riboflavin/UVB (Mirasol-like process). Modifications were identified and quantified by liquid chromatography coupled to tandem mass spectrometry. Cysteine-containing peptides formed disulfide bridges (R-SS-R, -2 Da; favored following amotosalen/UVA), sulfenic and sulfonic acids (R-SOH, +16 Da, R-SO3H, +48 Da, favored following riboflavin/UVB) upon treatment and the other amino acids exhibited different oxidations revealed by mass shifts from +4 to +34 Da involving different mechanisms; no photoadducts were detected. These amino acids were not equally affected by the PRT and the combination riboflavin/UVB generated more oxidation than amotosalen/UVA. This work identifies the different types and sites of peptide oxidations under the photochemical treatments and demonstrates that the two PRT may behave differently. The potential impact on proteins and platelet functions may thus be PRT-dependent.
Mots-clé
Anti-Infective Agents/pharmacology, Blood Platelets/microbiology, Chromatography, Liquid/methods, Oxidation-Reduction/drug effects, Oxidation-Reduction/radiation effects, Peptides/analysis, Peptides/chemistry, Photosensitizing Agents/pharmacology, Psoralens/pharmacology, Tandem Mass Spectrometry/methods, Ultraviolet Rays
Pubmed
Web of science
Création de la notice
09/11/2014 23:33
Dernière modification de la notice
03/03/2018 16:04
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