Article: article from journal or magazin.
Brain region mapping using global metabolomics.
Chemistry and Biology
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Historically, studies of brain metabolism have been based on targeted analyses of a limited number of metabolites. Here we present an untargeted mass spectrometry-based metabolomic strategy that has successfully uncovered differences in a broad array of metabolites across anatomical regions of the mouse brain. The NSG immunodeficient mouse model was chosen because of its ability to undergo humanization leading to numerous applications in oncology and infectious disease research. Metabolic phenotyping by hydrophilic interaction liquid chromatography and nanostructure imaging mass spectrometry revealed both water-soluble and lipid metabolite patterns across brain regions. Neurochemical differences in metabolic phenotypes were mainly defined by various phospholipids and several intriguing metabolites including carnosine, cholesterol sulfate, lipoamino acids, uric acid, and sialic acid, whose physiological roles in brain metabolism are poorly understood. This study helps define regional homeostasis for the normal mouse brain to give context to the reaction to pathological events.
Animals, Brain/anatomy & histology, Brain/metabolism, Brain Mapping, Chromatography, Affinity, Chromatography, High Pressure Liquid, Cluster Analysis, Hydrophobic and Hydrophilic Interactions, Interleukin Receptor Common gamma Subunit/deficiency, Interleukin Receptor Common gamma Subunit/genetics, Metabolomics, Mice, Mice, Inbred NOD, Mice, Knockout, Mice, SCID, Nanostructures/chemistry, Nuclear Magnetic Resonance, Biomolecular, Spectrometry, Mass, Electrospray Ionization
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