Analysis of teichoic acid biosynthesis regulation reveals that the extracytoplasmic function sigma factor sigmaM is induced by phosphate depletion in Bacillus subtilis W23.

Détails

ID Serval
serval:BIB_37326
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Analysis of teichoic acid biosynthesis regulation reveals that the extracytoplasmic function sigma factor sigmaM is induced by phosphate depletion in Bacillus subtilis W23.
Périodique
Microbiology
Auteur(s)
Minnig K., Lazarevic V., Soldo B., Mauël C.
ISSN
1350-0872[print], 1350-0872[linking]
Statut éditorial
Publié
Date de publication
2005
Volume
151
Numéro
Pt 9
Pages
3041-3049
Langue
anglais
Résumé
The expression of the Bacillus subtilis W23 tar genes specifying the biosynthesis of the major wall teichoic acid, the poly(ribitol phosphate), was studied under phosphate limitation using lacZ reporter fusions. Three different regulation patterns can be deduced from these beta-galactosidase activity data: (i) tarD and tarL gene expression is downregulated under phosphate starvation; (ii) tarA and, to a minor extent, tarB expression after an initial decrease unexpectedly increases; and (iii) tarO is not influenced by phosphate concentration. To dissect the tarA regulatory pattern, its two promoters were analysed under phosphate limitation: The P(tarA)-ext promoter is repressed under phosphate starvation by the PhoPR two-component system, whereas, under the same conditions, the P(tarA)-int promoter is upregulated by the action of an extracytoplasmic function (ECF) sigma factor, sigma(M). In contrast to strain 168, sigma(M) is activated in strain W23 in phosphate-depleted conditions, a phenomenon indirectly dependent on PhoPR, the two-component regulatory system responsible for the adaptation to phosphate starvation. These results provide further evidence for the role of sigma(M) in cell-wall stress response, and suggest that impairment of cell-wall structure is the signal activating this ECF sigma factor.
Mots-clé
Bacillus subtilis/genetics, Bacillus subtilis/metabolism, Bacterial Proteins, Cell Wall/metabolism, Gene Expression Regulation, Bacterial, Molecular Sequence Data, Phosphates/deficiency, Phosphates/metabolism, Sigma Factor/metabolism, Teichoic Acids/biosynthesis, Teichoic Acids/genetics, Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2007 13:35
Dernière modification de la notice
08/05/2019 17:04
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