Bacillus subtilis alpha-phosphoglucomutase is required for normal cell morphology and biofilm formation.

Détails

ID Serval
serval:BIB_37324
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Bacillus subtilis alpha-phosphoglucomutase is required for normal cell morphology and biofilm formation.
Périodique
Applied and Environmental Microbiology
Auteur(s)
Lazarevic V., Soldo B., Médico N., Pooley H., Bron S., Karamata D.
ISSN
0099-2240[print], 0099-2240[linking]
Statut éditorial
Publié
Date de publication
2005
Volume
71
Numéro
1
Pages
39-45
Langue
anglais
Résumé
Mutations designated gtaC and gtaE that affect alpha-phosphoglucomutase activity required for interconversion of glucose 6-phosphate and alpha-glucose 1-phosphate were mapped to the Bacillus subtilis pgcA (yhxB) gene. Backcrossing of the two mutations into the 168 reference strain was accompanied by impaired alpha-phosphoglucomutase activity in the soluble cell extract fraction, altered colony and cell morphology, and resistance to phages phi29 and rho11. Altered cell morphology, reversible by additional magnesium ions, may be correlated with a deficiency in the membrane glycolipid. The deficiency in biofilm formation in gtaC and gtaE mutants may be attributed to an inability to synthesize UDP-glucose, an important intermediate in a number of cell envelope biosynthetic processes.
Mots-clé
Bacillus Phages/physiology, Bacillus subtilis/enzymology, Bacillus subtilis/genetics, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Base Sequence, Biofilms/growth & development, Gene Expression Regulation, Bacterial, Glucosephosphates/biosynthesis, Molecular Sequence Data, Mutation, Phosphoglucomutase/chemistry, Phosphoglucomutase/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2007 13:35
Dernière modification de la notice
08/05/2019 17:04
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