Dpp spreading is required for medial but not for lateral wing disc growth.

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Version: Author's accepted manuscript
Serval ID
serval:BIB_36B5E117355B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dpp spreading is required for medial but not for lateral wing disc growth.
Journal
Nature
Author(s)
Harmansa S., Hamaratoglu F., Affolter M., Caussinus E.
ISSN
1476-4687 (Electronic)
ISSN-L
0028-0836
Publication state
Published
Issued date
11/2015
Peer-reviewed
Oui
Volume
527
Number
7578
Pages
317-322
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Drosophila Decapentaplegic (Dpp) has served as a paradigm to study morphogen-dependent growth control. However, the role of a Dpp gradient in tissue growth remains highly controversial. Two fundamentally different models have been proposed: the 'temporal rule' model suggests that all cells of the wing imaginal disc divide upon a 50% increase in Dpp signalling, whereas the 'growth equalization model' suggests that Dpp is only essential for proliferation control of the central cells. Here, to discriminate between these two models, we generated and used morphotrap, a membrane-tethered anti-green fluorescent protein (GFP) nanobody, which enables immobilization of enhanced (e)GFP::Dpp on the cell surface, thereby abolishing Dpp gradient formation. We find that in the absence of Dpp spreading, wing disc patterning is lost; however, lateral cells still divide at normal rates. These data are consistent with the growth equalization model, but do not fit a global temporal rule model in the wing imaginal disc.
Keywords
Animals, Body Patterning/physiology, Cell Proliferation, DNA-Binding Proteins/metabolism, Drosophila Proteins/metabolism, Drosophila melanogaster/cytology, Drosophila melanogaster/growth & development, Male, Repressor Proteins/metabolism, Signal Transduction, Single-Chain Antibodies, Transcription Factors/metabolism, Wings, Animal/cytology, Wings, Animal/growth & development
Pubmed
Web of science
Open Access
Yes
Create date
03/12/2015 9:43
Last modification date
20/08/2019 13:24
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