Release of tetanus toxoid from adjuvants and PLGA microspheres: how experimental set-up and surface adsorption fool the pattern.

Details

Serval ID
serval:BIB_3657A43CC45F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Release of tetanus toxoid from adjuvants and PLGA microspheres: how experimental set-up and surface adsorption fool the pattern.
Journal
Journal of Controlled Release : Official Journal of the Controlled Release Society
Author(s)
Johansen P., Corradin G., Merkle H.P., Gander B.
ISSN
0168-3659 (Print)
ISSN-L
0168-3659
Publication state
Published
Issued date
12/1998
Peer-reviewed
Oui
Volume
56
Number
1-3
Pages
209-217
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The classical adjuvants alum and Freund's Incomplete Adjuvant (IFA) are frequently used as references for the design of new adjuvants and antigen delivery systems, e.g., microspheres (MS). Poly(dl-lactic-co-glycolic acid) (PLGA) MS have been proposed for delivering antigen booster doses in vivo after a single injection. However, as antigen release kinetics from conventional adjuvants are generally unknown, it appears presumptuous to propose a desired antigen release pattern from PLGA MS. Therefore, we have studied the tetanus toxoid (Ttxd) in vitro release from alum, IFA formulations and MS in four different test systems. The results showed a stronger Ttxd association to alum than to IFA, and the release from both formulations lasted between 3-9 days. The total of ELISA-responsive antigen released was 60-85% of the actual dose. Both the total amount and the prolongation of release depended on the Ttxd dose. Furthermore, the incomplete in vitro release of Ttxd from the adjuvants and also from PLGA 50:50 MS was shown to be partly due to experimental conditions. Typically, Ttxd adsorbed on the glass vials used for the release test and also on the surface of the PLGA 50:50 MS, wherefrom it was released. In conclusion, the test system depending rate and quantity of release observed evidence the limitations of in vitro release data. Finally, for mimicking conventional vaccination schedules, i.e. injections typically at time points 0, 1, 3, and 12-24 months, PLGA MS should release antigen doses at the corresponding time points, and the release pulse should only last for a few days.
Keywords
Adjuvants, Immunologic/chemistry, Adsorption, Biocompatible Materials/administration & dosage, Biocompatible Materials/chemistry, Chemistry, Pharmaceutical, Delayed-Action Preparations, Kinetics, Lactic Acid/administration & dosage, Lactic Acid/chemistry, Microspheres, Polyglycolic Acid/administration & dosage, Polyglycolic Acid/chemistry, Polymers/administration & dosage, Polymers/chemistry, Surface Properties, Tetanus Toxoid/administration & dosage, Tetanus Toxoid/chemistry
Pubmed
Web of science
Create date
24/01/2008 14:55
Last modification date
20/08/2019 13:24
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