Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid identification of Beta-hemolytic streptococci.
Details
Serval ID
serval:BIB_35A3C4FB53E8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid identification of Beta-hemolytic streptococci.
Journal
Journal of Clinical Microbiology
ISSN
1098-660X (Electronic)
ISSN-L
0095-1137
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
49
Number
8
Pages
3004-3005
Language
english
Notes
Publication types: Comparative Study ; Evaluation Studies ; Journal ArticlePublication Status: ppublish
Abstract
This study was undertaken to evaluate matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the rapid identification of beta-hemolytic streptococci. We compared Bruker Biotyper 2.0 with Vitek2 coupled to the agglutination test. MALDI-TOF MS analysis of 386 beta-hemolytic streptococcal isolates yielded high-confidence identification to the species level for all 386 isolates. The Vitek2 gave high-confidence identification to the species level for 88% of Streptococcus agalactiae isolates (n = 269/306), 92% of Streptococcus pyogenes isolates (n = 48/52), and 39% of isolates of Streptococcus dysgalactiae serogroups C and G (n = 11/28).
Keywords
Bacteriological Techniques/methods, Humans, Sensitivity and Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods, Streptococcal Infections/diagnosis, Streptococcal Infections/microbiology, Streptococcus/chemistry, Streptococcus/classification
Pubmed
Open Access
Yes
Create date
09/01/2012 9:14
Last modification date
20/08/2019 13:23
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