Xenon detection in human blood: Analytical validation by accuracy profile and identification of critical storage parameters.

Détails

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Etat: Public
Version: Author's accepted manuscript
Licence: CC BY-NC-ND 4.0
ID Serval
serval:BIB_35807B8A121A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Xenon detection in human blood: Analytical validation by accuracy profile and identification of critical storage parameters.
Périodique
Journal of forensic and legal medicine
Auteur(s)
Frampas C., Ney J., Coburn M., Augsburger M., Varlet V.
ISSN
1878-7487 (Electronic)
ISSN-L
1752-928X
Statut éditorial
Publié
Date de publication
08/2018
Peer-reviewed
Oui
Volume
58
Pages
14-19
Langue
anglais
Notes
Publication types: Journal Article ; Validation Studies
Publication Status: ppublish
Résumé
Xenon is a rare, mostly inert, noble gas that has applications in a wide range of fields, including medicine. Xenon acts on the human body as a useful organ-protective and anesthetic agent and has also been previously studied for potential applications in fields such as optics, aerospace and medical imaging. Recently, it was discovered that xenon can boost erythropoietin production, and it has been used as a performance-enhancing agent in international sports competitions such as the Sochi Olympic Games. Therefore, screening methods to detect the misuse of xenon by analysis of biological samples and to monitor anesthesia kinetics and efficiency are being investigated. The aim of this study was to develop and validate an analytical method to detect xenon in blood samples using gas chromatography coupled to tandem mass spectrometry (GC-MS/MS). Preliminary studies were conducted to determine the best parameters for chromatography and mass spectrometry for xenon. The analysis was performed using the multiple reaction monitoring (MRM) mode using the transitions m/z 129 → 129, 131 → 131 for xenon and 84 → 84, 86 → 86 for krypton, which was chosen as the internal standard. The LOD of GC-MS/MS was found to be 52 pmol on-column. Calibration lines and controls were made to obtain an accuracy profile at a range of 2.08-104 nmol with a β-expectation tolerance interval set at 80% and the acceptability limit set at ±30%. From the accuracy profile, the LOQ of 15 nmol on-column for the range of 2.08-104 nmol was obtained. The method was validated according to the guidelines of the French Society of Pharmaceutical Sciences and Techniques. The detection method was finally validated using blood from test persons subjected to a 15% or 30% xenon mixture with pure oxygen and air for 45 min. Even though the probes were already used for other projects, it was still possible to detect xenon.
Mots-clé
Anesthetics, Inhalation/blood, Chromatography, Gas, Doping in Sports, Humans, Limit of Detection, Substance Abuse Detection/methods, Tandem Mass Spectrometry, Xenon/blood, Accuracy profile, Anesthesia, Anti-doping, Blood analysis, GC-MS/MS, Xenon
Pubmed
Web of science
Création de la notice
24/04/2018 9:06
Dernière modification de la notice
20/08/2019 14:23
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