An exogenous mouse mammary tumor virus with properties of Mls-1a (Mtv-7).

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Serval ID
serval:BIB_34B1C10DA8A9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
An exogenous mouse mammary tumor virus with properties of Mls-1a (Mtv-7).
Journal
Journal of Experimental Medicine
Author(s)
Held W., Shakhov A.N., Waanders G., Scarpellino L., Luethy R., Kraehenbuhl J.P., MacDonald H.R., Acha-Orbea H.
ISSN
0022-1007 (Print)
ISSN-L
0022-1007
Publication state
Published
Issued date
1992
Volume
175
Number
6
Pages
1623-1633
Language
english
Abstract
The classical minor lymphocyte stimulating (Mls) antigens, which induce a strong primary T cell response in vitro, are closely linked to endogenous copies of mouse mammary tumor viruses (MMTV). Expression of Mls genes leads to clonal deletion of T cell subsets expressing specific T cell receptor (TCR) V beta chains. We describe the isolation and characterization of a new exogenous (infectious) MMTV with biological properties similar to the Mls antigen Mls-1a. In vivo administration of either Mls-1a-expressing B cells or the infectious MMTV (SW) led to an increase of T cells expressing V beta 6 followed by their deletion. Surprisingly, different kinetics of deletion were observed with the exogenous virus depending upon the route of infection. Infection through the mucosa led to a slow deletion of V beta 6+ T cells, whereas deletion was rapid after subcutaneous infection. Sequence analysis of the open reading frames in the 3' long terminal repeat of both this exogenous MMTV (SW) and of Mtv-7 (which is closely linked to Mls-1a) revealed striking similarities, particularly in the COOH terminus, which has been implicated in TCR V beta recognition. The identification of an infectious MMTV with the properties of a strong Mls antigen provides a new, powerful tool to study immunity and tolerance in vivo.
Keywords
Amino Acid Sequence, Animals, Antibodies, Monoclonal, Base Sequence, Cloning, Molecular, Female, Genes, Viral, Lymph Nodes/immunology, Mammary Tumor Virus, Mouse/genetics, Mammary Tumor Virus, Mouse/isolation &amp, purification, Mice, Mice, Inbred BALB C, Mice, Inbred C3H, Mice, Inbred Strains, Milk/microbiology, Minor Lymphocyte Stimulatory Antigens/analysis, Minor Lymphocyte Stimulatory Antigens/genetics, Molecular Sequence Data, Oligodeoxyribonucleotides, Polymerase Chain Reaction/methods, Receptors, Antigen, T-Cell/genetics, Sequence Homology, Nucleic Acid, Species Specificity, T-Lymphocytes/immunology, Thymus Gland/immunology
Pubmed
Web of science
Create date
17/01/2008 15:24
Last modification date
20/08/2019 13:21
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