Determination and distribution of clotiapine (Entumine) in human plasma, post-mortem blood and tissue samples from clotiapine-treated patients and from autopsy cases

Details

Serval ID
serval:BIB_349D98D54E8D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Determination and distribution of clotiapine (Entumine) in human plasma, post-mortem blood and tissue samples from clotiapine-treated patients and from autopsy cases
Journal
Forensic Science International
Author(s)
Sporkert  Frank, Augsburger  Marc, Giroud  Christian, Brossard  Clarisse, Eap  Chin-Bin, Mangin  Patrice
ISSN
0379-0738
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
170
Number
2/3
Pages
193-199
Language
english
Notes
SAPHIRID:62855
Abstract
The antipsychotic drug clotiapine (Entumine) has been marketed for more than 35 years, however there is little published data on the therapeutic and toxic concentrations of this drug. To fill this gap, two rapid and sensitive methods were developed for the determination of clotiapine (2-chloro-11-(4-methyl-1-piperazinyl)dibenzo-[b,f][1,4]-thiazepine), in human plasma and post-mortem blood and tissue samples. After simple liquid-liquid extraction at pH 9.5 with n-hexane/dichloromethane (85/15, v/v), clotiapine was quantitated by HPLC-DAD and by GC-NPD. The calibration curve was linear between 10 and 1000 microg/L. The limit of detection (LOD) and the limit of quantification (LOQ) were found to be 2 and 6 microg/L for the GC-NPD method and 5 and 15 microg/L for the HPLC-method, respectively. These methods were applied to 12 plasma samples from patients treated with clotiapine, to seven autopsy cases and to one case of driving under the influence of drugs (DUID). Concentrations ranged for the clotiapine-treated patients between 6 and 155 microg/L (mean 46 microg/L), and for the autopsy cases between 22 and 341 microg/L (mean 123 microg/L).
Pubmed
Web of science
Create date
05/02/2008 13:39
Last modification date
20/08/2019 13:21
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