Article: article from journal or magazin.
Evaluation of MRSA-Screen, a simple anti-PBP 2a slide latex agglutination kit, for rapid detection of methicillin resistance in Staphylococcus aureus.
Journal of Clinical Microbiology
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
The MRSA-Screen test (Denka Seiken Co., Ltd., Tokyo, Japan), consisting of a slide latex agglutination kit that detects PBP 2a with a monoclonal antibody, was blindly compared to the oxacillin disk diffusion test, the oxacillin-salt agar screen, and PCR of the mecA gene for the detection of methicillin resistance in Staphylococcus aureus. A total of 120 methicillin-susceptible S. aureus (MSSA) and 80 methicillin-resistant S. aureus (MRSA) isolates, defined by the absence or presence of the mecA gene, respectively, were tested. The MRSA-Screen test, the oxacillin disk diffusion test, and the oxacillin-salt agar screening test showed sensitivities of 100, 61.3, and 82.5% and specificities of 99.2, 96.7, and 98.3%, respectively. We conclude that the MRSA-Screen is a very accurate, reliable, and fast test (15 min) for differentiation of MRSA from MSSA colonies on agar plates.
Antibodies, Monoclonal/immunology, Bacterial Proteins/genetics, Carrier Proteins/analysis, Carrier Proteins/immunology, Hexosyltransferases, Humans, Latex Fixation Tests, Methicillin Resistance, Muramoylpentapeptide Carboxypeptidase/analysis, Muramoylpentapeptide Carboxypeptidase/immunology, Oxacillin/pharmacology, Penicillin-Binding Proteins, Peptidyl Transferases, Reagent Kits, Diagnostic, Sensitivity and Specificity, Staphylococcus aureus/drug effects
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