Article: article from journal or magazin.
RT-PCR diagnosis of patients with acute nonlymphocytic leukemia and inv(16)(p13q22) and identification of new alternative splicing in CBFB-MYH11 transcripts.
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
As acute nonlymphocytic leukemia (ANLL) with inv(16) (p13q22) or t(16;16)(p13;q22) has been shown to result from the fusion of transcription factor subunit core binding factor (CBFB) to a myosin heavy chain (MYH11), we sought to design methods to detect this rearrangement using reverse transcriptase-polymerase chain reaction (RT-PCR). In all of 27 inv(16)(p13q22) and four t(16;16)(p13;q22) cases tested, a chimeric CBFB-MYH11 transcript coding for an in-frame fusion protein was detected. In a more extensive RT-PCR analysis with different primer pairs, we detected a second new chimeric CBFB-MYH11 transcript in 10 of 11 patients tested. The CBFB-MYH11 reading frame of the second transcript was maintained in one patient but not in the others. We show that the different CBFB-MYH11 transcripts in one patient arise from alternative splicing. Translation of the transcript in which the CBFB-MYH11 reading frame is not maintained leads to a slightly truncated CBFB protein.
Adolescent, Adult, Aged, Base Sequence, Child, Chromosomes, Human, Pair 16, Core Binding Factors, DNA-Binding Proteins, Female, Gene Expression Regulation, Leukemic, Humans, Inversion, Chromosome, Leukemia, Myeloid, Acute, Male, Middle Aged, Molecular Sequence Data, Myosins, Neoplasm Proteins, Oncogene Proteins, Fusion, Polymerase Chain Reaction, Protein Biosynthesis, RNA Splicing, Transcription Factors, Transcription, Genetic
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