Article: article from journal or magazin.
Cutting edge: IL-7 regulates the peripheral pool of adult ROR gamma+ lymphoid tissue inducer cells.
Journal of immunology
During fetal life, CD4(+)CD3(-) lymphoid tissue inducer (LTi) cells are required for lymph node and Peyer's patch development in mice. In adult animals, CD4(+)CD3(-) cells are found in low numbers in lymphoid organs. Whether adult CD4(+)CD3(-) cells are LTi cells and are generated and maintained through cytokine signals has not been directly addressed. In this study we show that adult CD4(+)CD3(-) cells adoptively transferred into neonatal CXCR5(-/-) mice induced the formation of intestinal lymphoid tissues, demonstrating for the first time their bona fide LTi function. Increasing IL-7 availability in wild-type mice either by IL-7 transgene expression or treatment with IL-7/anti-IL-7 complexes increased adult LTi cell numbers through de novo generation from bone marrow cells and increased the survival and proliferation of LTi cells. Our observations demonstrate that adult CD4(+)lineage(-) cells are LTi cells and that the availability of IL-7 determines the size of the adult LTi cell pool.
Animals, Antigens, CD3/metabolism, CD4-Positive T-Lymphocytes/cytology, CD4-Positive T-Lymphocytes/immunology, Cell Differentiation/genetics, Cell Differentiation/immunology, Cells, Cultured, Fetus/cytology, Fetus/immunology, Interleukin-7/biosynthesis, Interleukin-7/deficiency, Lymphangiogenesis/genetics, Lymphangiogenesis/immunology, Lymphoid Tissue/cytology, Lymphoid Tissue/immunology, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Receptors, Retinoic Acid/biosynthesis, Receptors, Retinoic Acid/deficiency, Receptors, Thyroid Hormone/biosynthesis, Receptors, Thyroid Hormone/deficiency, Spleen/cytology, Spleen/immunology, T-Lymphocyte Subsets/cytology, T-Lymphocyte Subsets/immunology
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