Long-term stability and age-dependence of six regulatory serum proteins.
Details
Serval ID
serval:BIB_33DF2397AA31
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Long-term stability and age-dependence of six regulatory serum proteins.
Journal
Biomarkers in medicine
ISSN
1752-0371 (Electronic)
ISSN-L
1752-0363
Publication state
Published
Issued date
05/2022
Peer-reviewed
Oui
Volume
16
Number
7
Pages
511-521
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Aim: The development of biomarker-based diagnostic procedures often relies on samples stored for several years. We aimed to investigate the influence of storage time and patient age on six neuroregulatory and immunoregulatory serum biomarkers. Materials & methods: We quantified six biomarkers in serum from 151 individuals using ELISA. Serum was stored at -80°C for up to 9.5 years. Results: When associating storage time with biomarker values, BDNF, VEGF-A and TGF-β1 showed a significant increase over time; IGF-1, MCP-1 and IL-18 did not. Associating participant age with biomarkers, only IL-18 in Alzheimer's disease patients showed a significant increase. Conclusion: Storage time can influence results of biomarkers in human serum. This needs to be considered when assessing samples stored for several years.
Keywords
Alzheimer Disease, Biomarkers, Enzyme-Linked Immunosorbent Assay, Humans, Alzheimer’s disease, age-dependence, biobanking, biomarkers, blood serum, cognitive impairment, long-term storage, neuroinflammation, neuroprotection, storage time
Pubmed
Web of science
Create date
21/03/2022 9:41
Last modification date
04/11/2023 8:09
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