Establishing two-dimensional gels for the analysis of Leishmania proteomes.

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Serval ID
serval:BIB_33D358FBBC78
Type
Article: article from journal or magazin.
Collection
Publications
Institution
UNIL/CHUV
Title
Establishing two-dimensional gels for the analysis of Leishmania proteomes.
Journal
Proteomics
Author(s)
Acestor N., Masina S., Walker J., Saravia N.G., Fasel N., Quadroni M.
ISSN
1615-9853 (Print)
ISSN-L
1615-9853
Publication state
Published
Issued date
07/2002
Peer-reviewed
Oui
Volume
2
Number
7
Pages
877-879
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Several different sample preparation methods for two-dimensional electrophoresis (2-DE) analysis of Leishmania parasites were compared. From this work, we were able to identify a solubilization method using Nonidet P-40 as detergent, which was simple to follow, and which produced 2-DE gels of high resolution and reproducibility.
Keywords
Animals, Electrophoresis, Gel, Two-Dimensional/methods, Leishmania/chemistry, Leishmania/genetics, Molecular Weight, Proteome/chemistry, Protozoan Proteins/analysis
OAI-PMH
oai:serval.unil.ch:BIB_33D358FBBC78
DOI
10.1002/1615-9861(200207)2:7<877::AID-PROT877>3.0.CO;2-D
Pubmed
12124933
Web of science
000177056800010
Create date
24/01/2008 15:02
Last modification date
09/04/2024 6:14
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