Article: article from journal or magazin.
Establishing two-dimensional gels for the analysis of Leishmania proteomes.
Several different sample preparation methods for two-dimensional electrophoresis (2-DE) analysis of Leishmania parasites were compared. From this work, we were able to identify a solubilization method using Nonidet P-40 as detergent, which was simple to follow, and which produced 2-DE gels of high resolution and reproducibility.
Animals, Electrophoresis, Gel, Two-Dimensional/methods, Leishmania/chemistry, Leishmania/genetics, Molecular Weight, Proteome/chemistry, Protozoan Proteins/analysis
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