Deregulated MHC class II transactivator expression leads to a strong Th2 bias in CD4+ T lymphocytes.

Détails

ID Serval
serval:BIB_33B0D6CCD5D0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Deregulated MHC class II transactivator expression leads to a strong Th2 bias in CD4+ T lymphocytes.
Périodique
Journal of Immunology
Auteur(s)
Otten L.A., Tacchini-Cottier F., Lohoff M., Annunziato F., Cosmi L., Scarpellino L., Louis J., Steimle V., Reith W., Acha-Orbea H.
ISSN
0022-1767[print], 0022-1767[linking]
Statut éditorial
Publié
Date de publication
2003
Volume
170
Numéro
3
Pages
1150-1157
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Résumé
The MHC class II (MHC-II) transactivator (CIITA) is the master transcriptional regulator of genes involved in MHC-II-restricted Ag presentation. Fine tuning of CIITA gene expression determines the cell type-specific expression of MHC-II genes. This regulation is achieved by the selective usage of multiple CIITA promoters. It has recently been suggested that CIITA also contributes to Th cell differentiation by suppressing IL-4 expression in Th1 cells. In this study, we show that endogenous CIITA is expressed at low levels in activated mouse T cells. Importantly CIITA is not regulated differentially in murine and human Th1 and Th2 cells. Ectopic expression of a CIITA transgene in multiple mouse cell types including T cells, does not interfere with normal development of CD4(+) T cells. However, upon TCR activation the CIITA transgenic CD4(+) T cells preferentially differentiate into IL-4-secreting Th2-type cells. These results imply that CIITA is not a direct Th1-specific repressor of the IL-4 gene and that tight control over the expression of CIITA and MHC-II is required to maintain the normal balance between Th1 and Th2 responses.
Mots-clé
Adult, Animals, CD4-Positive T-Lymphocytes/cytology, CD4-Positive T-Lymphocytes/immunology, Cell Differentiation/genetics, Cell Differentiation/immunology, Cytokines/biosynthesis, Gene Expression Regulation/immunology, Genes, MHC Class II/genetics, Humans, Lymphocyte Activation/genetics, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Transgenic, Nuclear Proteins, Species Specificity, Th1 Cells/immunology, Th1 Cells/metabolism, Th2 Cells/immunology, Th2 Cells/metabolism, Trans-Activators/biosynthesis, Trans-Activators/deficiency
Pubmed
Web of science
Création de la notice
24/01/2008 16:08
Dernière modification de la notice
20/08/2019 14:19
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