Optimizing Photobiomodulation Radiometric and Spectral Parameters In Vitro to Enhance Angiogenesis and Mitochondrial Function.

Details

Serval ID
serval:BIB_33AAD43E2DFA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Optimizing Photobiomodulation Radiometric and Spectral Parameters In Vitro to Enhance Angiogenesis and Mitochondrial Function.
Journal
International journal of molecular sciences
Author(s)
Joniová J., Gregor A., Lambelet M., Déglise S., Allagnat F., Wagnières G.
ISSN
1422-0067 (Electronic)
ISSN-L
1422-0067
Publication state
Published
Issued date
26/12/2024
Peer-reviewed
Oui
Volume
26
Number
1
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Photobiomodulation (PBM) therapy, a therapeutic approach utilizing low-level light, has garnered significant attention for its potential to modulate various biological processes. This study aimed at optimizing and investigating the effects of PBM on angiogenesis and mitochondrial metabolic activity. In vitro experiments using human umbilical vein endothelial cells (HUVECs) and vascular smooth muscle cells (VSMCs) were performed to assess PBM's impacts on cell migration, proliferation, endogenous protoporphyrin IX production, mitochondrial membrane potential, Rhodamine 123 fluorescence lifetime, mitochondrial morphology, and oxygen consumption. Our findings demonstrated that the PBM approach significantly stimulates HUVECs and VSMCs, highlighting the importance of precise light dosimetry for optimal outcomes. Interestingly, our results indicate that in our conditions, the optimal radiometric and spectral parameters are similar for HUVECs and VSMCs for the different endpoints mentioned above. In conclusion, our study strongly suggests that PBM holds promise as a therapeutic intervention for conditions characterized by impaired angiogenesis, such as wound healing, ischemia, and cardiovascular disease. Further research is necessary to fully elucidate the underlying mechanisms and optimize the radiometric and spectral parameters for clinical applications.
Keywords
Humans, Mitochondria/metabolism, Mitochondria/radiation effects, Human Umbilical Vein Endothelial Cells/metabolism, Low-Level Light Therapy/methods, Cell Proliferation/radiation effects, Cell Movement/radiation effects, Neovascularization, Physiologic/radiation effects, Membrane Potential, Mitochondrial/radiation effects, Muscle, Smooth, Vascular/metabolism, Muscle, Smooth, Vascular/cytology, Muscle, Smooth, Vascular/radiation effects, Protoporphyrins/metabolism, Protoporphyrins/pharmacology, Radiometry, Myocytes, Smooth Muscle/metabolism, Myocytes, Smooth Muscle/radiation effects, Myocytes, Smooth Muscle/cytology, Oxygen Consumption/radiation effects, Cells, Cultured, Angiogenesis, PBM, angiogenesis, cell migration, endothelial cells, mitochondrial metabolic activity, photobiomodulation, protoporphyrin IX
Pubmed
Web of science
Open Access
Yes
Create date
17/01/2025 14:54
Last modification date
18/01/2025 7:08
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