In vitro and in vivo effectiveness of an innovative silver-copper nanoparticle coating of catheters to prevent methicillin-resistant Staphylococcus aureus infection

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Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_331CBDC800C0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
In vitro and in vivo effectiveness of an innovative silver-copper nanoparticle coating of catheters to prevent methicillin-resistant Staphylococcus aureus infection
Périodique
Antimicrobial Agents and Chemotherapy
Auteur(s)
Ballo Myriam K.S., Rtimi Sami, Pulgarin César, Hopf Nancy, Berthet Aurélie, Kiwi John, Moreillon Philippe, Entenza José M., Bizzini Alain
ISSN
1098-6596 (Electronic)
ISSN-L
0066-4804
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
60
Numéro
9
Pages
5349-5356
Langue
anglais
Résumé
In this study, silver/copper (Ag/Cu)-coated catheters were investigated for their efficacy in preventing methicillin-resistant Staphylococcus aureus (MRSA) infection in vitro and in vivoAg and Cu were sputtered (67/33% atomic ratio) on polyurethane catheters by Direct Current Magnetron Sputtering. In vitro, Ag/Cu-coated and uncoated catheters were immersed in PBS or rat plasma and exposed to 10(4)-10(8) CFU/ml of MRSA ATCC 43300. In vivo, Ag/Cu-coated and uncoated catheters were placed in the jugular vein of rats. Close by, MRSA (10(7) CFU/ml) was inoculated in the tail vein. Catheters were removed 48 h later and cultured.In vitro, Ag/Cu-coated catheters pre-incubated in PBS and exposed to 10(4)-10(7) CFU/ml, prevented the adherence of MRSA (0-12% colonization) compared to uncoated catheters (50-100% colonization; P< 0.005), Ag/Cu-coated catheters retained their activity (0-20% colonization) when pre-incubated in rat plasma while colonization of uncoated catheters increased (83-100%; P< 0.005). Ag/Cu-coating protection diminished with 10(8) CFU/ml in both PBS and plasma (50-100% colonization). In vivo, Ag/Cu-coated catheters reduced the incidence of catheter infection compared to uncoated catheters (57% vs 79%, respectively; P= 0.16) and bacteremia (31% vs 68%, respectively; P< 0.05). Scanning electron microscopy of explanted catheters suggests that the suboptimal activity of Ag/Cu catheters in vivo was due to the formation of a dense fibrin sheath over their surface.Ag/Cu-coated catheters have a potential for preventing MRSA infections. Their activity might be improved by limiting plasma protein adsorption on their surface.
Mots-clé
Catheters, Methicillin-Resistant Staphylococcus aureus, Silver, Copper
Pubmed
Web of science
Open Access
Oui
Création de la notice
01/07/2016 10:16
Dernière modification de la notice
08/05/2019 16:48
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