Neutralizing and protective antibodies directed against vaccinia virus envelope antigens.

Details

Serval ID
serval:BIB_32186C574580
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Neutralizing and protective antibodies directed against vaccinia virus envelope antigens.
Journal
Virology
Author(s)
Galmiche M.C., Goenaga J., Wittek R., Rindisbacher L.
ISSN
0042-6822[print], 0042-6822[linking]
Publication state
Published
Issued date
02/1999
Volume
254
Number
1
Pages
71-80
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The infection mechanism of vaccinia virus is largely unknown. Neither the attachment protein of extracellular enveloped virus (EEV), the biologically relevant infectious form of the virus, nor its cellular receptor has been identified. Surprisingly, all former attempts using antibodies to block EEV infection of cells in vitro had failed. Here, we report the production of an anti-envelope hyperimmune serum with EEV neutralizing activity and show that a polyclonal antiserum against the extraviral domain of protein B5R also inhibited EEV infection. In vivo, mice vaccinated with B5R protein were protected against a lethal vaccinia virus challenge. This protectivity is likely to be mediated by neutralizing antibodies. Protein A33R, but not A34R and A36R, also proved to be protective in active and passive vaccination experiments. However, in contrast to B5R, A33R protectivity did not correlate with antibody titers. Because anti-A33R antibodies did not neutralize EEV in vitro, the protectivity mediated by A33R protein probably involves a mechanism different from simple antibody binding. Taken together, our results suggest that antibodies to a specific protective epitope or epitopes on protein B5R are able to prevent EEV infection. The protein encoded by the B5R gene is therefore likely to play a crucial role in the initial steps of vaccinia virus infection-binding to a host cell and entry into its cytoplasm.
Keywords
Animals, Antibodies, Viral/biosynthesis, Antibodies, Viral/immunology, Antigens, Viral/genetics, Antigens, Viral/immunology, Cell Line, DNA, Viral/immunology, Glycoproteins/genetics, Glycoproteins/immunology, Humans, Membrane Glycoproteins/genetics, Membrane Glycoproteins/immunology, Mice, Neutralization Tests, Rabbits, Tumor Cells, Cultured, Vaccination, Vaccines, DNA/immunology, Vaccinia/prevention &amp, control, Vaccinia virus/immunology, Viral Envelope Proteins/genetics, Viral Envelope Proteins/immunology, Viral Vaccines/immunology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 10:43
Last modification date
20/08/2019 13:17
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