Melanocytes and pigmentation are affected in dopachrome tautomerase knockout mice.

Details

Serval ID
serval:BIB_31412
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Melanocytes and pigmentation are affected in dopachrome tautomerase knockout mice.
Journal
Molecular and Cellular Biology
Author(s)
Guyonneau L., Murisier F., Rossier A., Moulin A., Beermann F.
ISSN
0270-7306
Publication state
Published
Issued date
2004
Volume
24
Number
8
Pages
3396-3403
Language
english
Notes
Publication types: Journal Article
Abstract
The tyrosinase family comprises three members, tyrosinase (Tyr), tyrosinase-related protein 1 (Tyrp1), and dopachrome tautomerase (Dct). Null mutations and deletions at the Tyr and Tyrp1 loci are known and phenotypically affect coat color due to the absence of enzyme or intracellular mislocalization. At the Dct locus, three mutations are known that lead to pigmentation phenotype. However, these mutations are not null mutations, and we therefore set out to generate a null allele at the Dct gene locus by removing exon 1 of the mouse Dct gene. Mice deficient in Dct [Dct(tm1(Cre)Bee)] lack Dct mRNA and dopachrome tautomerase protein. They are viable and do not show any abnormalities in Dct-expressing sites such as skin, retinal pigment epithelium, or brain. However, the mice show a diluted coat color phenotype, which is due to reduced melanin content in hair. Primary melanocytes from Dct knockout mice are viable in culture and show a normal distribution of tyrosinase and tyrosinase-related protein 1. In comparison to the knockout, the slaty mutation (Dct(slt)/Dct(slt)) has less melanin and affects growth of primary melanocytes severely. In summary, we have generated a knockout of the Dct gene in mice with effects restricted to pigment production and coat color.
Keywords
Animals, Brain/cytology, Brain/metabolism, Cells, Cultured, Eye/cytology, Eye/metabolism, Gene Targeting, Intramolecular Oxidoreductases/genetics, Intramolecular Oxidoreductases/metabolism, Melanocytes/cytology, Melanocytes/metabolism, Membrane Glycoproteins, Mice, Mice, Inbred C57BL, Mice, Knockout, Monophenol Monooxygenase/genetics, Monophenol Monooxygenase/metabolism, Mutation, Oxidoreductases, Phenotype, Pigmentation/physiology, Proteins/genetics, Proteins/metabolism, Skin/cytology, Skin/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:30
Last modification date
20/08/2019 13:16
Usage data