The heterotetrameric architecture of the epithelial sodium channel (ENaC).

Détails

ID Serval
serval:BIB_30B0416318EB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The heterotetrameric architecture of the epithelial sodium channel (ENaC).
Périodique
EMBO Journal
Auteur(s)
Firsov D., Gautschi I., Merillat A.M., Rossier B.C., Schild L.
ISSN
0261-4189 (Print)
ISSN-L
0261-4189
Statut éditorial
Publié
Date de publication
1998
Volume
17
Numéro
2
Pages
344-352
Langue
anglais
Résumé
The epithelial sodium channel (ENaC) is a key element for the maintenance of sodium balance and the regulation of blood pressure. Three homologous ENaC subunits (alpha, beta and gamma) assemble to form a highly Na+-selective channel. However, the subunit stoichiometry of ENaC has not yet been solved. Quantitative analysis of cell surface expression of ENaC alpha, beta and gamma subunits shows that they assemble according to a fixed stoichiometry, with alpha ENaC as the most abundant subunit. Functional assays based on differential sensitivities to channel blockers elicited by mutations tagging each alpha, beta and gamma subunit are consistent with a four subunit stoichiometry composed of two alpha, one beta and one gamma. Expression of concatameric cDNA constructs made of different combinations of ENaC subunits confirmed the four subunit channel stoichiometry and showed that the arrangement of the subunits around the channel pore consists of two alpha subunits separated by beta and gamma subunits.
Mots-clé
Amiloride/pharmacology, Animals, Cell Membrane/metabolism, Epithelial Sodium Channel, Ligands, Macromolecular Substances, Mutagenesis, Oocytes/metabolism, Polymerase Chain Reaction, Rats, Sodium Channel Blockers, Sodium Channels/biosynthesis, Sodium Channels/chemistry, Xenopus
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 13:32
Dernière modification de la notice
20/08/2019 14:15
Données d'usage