Article: article from journal or magazin.
Insulinoma associated with a case of multiple endocrine neoplasia type I: Functional somatostatin receptors and abnormal glucose-induced insulin secretion.
Publication types: Case Reports ; Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
A sporadic case of multiple endocrine neoplasia type I with coexisting insulinoma and hyperparathyroidism was investigated in vivo and in vitro. The insulinoma was localized by somatostatin receptor scintigraphy and these receptors were functionally active. Octreotide administration decreased the basal insulin and glucagon secretion by 90 and 46%, respectively. Immunocytochemistry of the insulinoma tissue was positive for insulin, chromogranin A and neuropeptide Y. The insulinoma cells were also isolated and cultured in vitro. Incubation experiments revealed that a low glucose concentration (1 mmol/l) was sufficient to increase cytosolic free calcium and to produce a maximal glucose-induced insulin release. Northern blot analysis of RNA obtained from the tumor showed a high abundance of the low Km glucose transporter GLUT1 but no transcript for the high Km glucose transporter GLUT2. The abnormal distribution of glucose transporters probably relates to the abnormal glucose sensing of insulinoma cells, and explains their sustained insulin secretion at low glucose concentrations. Whether these abnormalities share a pathogenetic link with the presence of functionally active somatostatin receptors remains to be elucidated.
Actins, Adolescent, Dose-Response Relationship, Drug, Follow-Up Studies, Gene Expression Regulation, Neoplastic, Glucagon, Glucose, Glucose Transporter Type 1, Hormones, Humans, Hyperparathyroidism, Immunohistochemistry, Injections, Intravenous, Insulin, Insulinoma, Male, Monosaccharide Transport Proteins, Multiple Endocrine Neoplasia Type 1, Octreotide, Pancreatic Neoplasms, Parathyroid Neoplasms, RNA, Messenger, Receptors, Somatostatin, Tomography, Emission-Computed, Single-Photon
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