Fast analysis of doping agents in urine by ultra-high-pressure liquid chromatography-quadrupole time-of-flight mass spectrometry. II: Confirmatory analysis.

Details

Serval ID
serval:BIB_2FB5B826A6D7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Fast analysis of doping agents in urine by ultra-high-pressure liquid chromatography-quadrupole time-of-flight mass spectrometry. II: Confirmatory analysis.
Journal
Journal of Chromatography. A
Author(s)
Badoud F., Grata E., Perrenoud L., Saugy M., Rudaz S., Veuthey J.L.
ISSN
1873-3778[electronic], 0021-9673[linking]
Publication state
Published
Issued date
2010
Volume
1217
Number
25
Pages
4109-4119
Language
english
Abstract
For doping control, analyses of samples are generally achieved in two steps: a rapid screening and, in the case of a positive result, a confirmatory analysis. A two-step methodology based on ultra-high-pressure liquid chromatography coupled to a quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) was developed to screen and confirm 103 doping agents from various classes (e.g., beta-blockers, stimulants, diuretics, and narcotics). The screening method was presented in a previous article as part I (i.e., Fast analysis of doping agents in urine by ultra-high-pressure liquid chromatography-quadrupole time-of-flight mass spectrometry. Part I: screening analysis). For the confirmatory method, basic, neutral and acidic compounds were extracted by a dedicated solid-phase extraction (SPE) in a 96-well plate format and detected by MS in the tandem mode to obtain precursor and characteristic product ions. The mass accuracy and the elemental composition of precursor and product ions were used for compound identification. After validation including matrix effect determination, the method was considered reliable to confirm suspect results without ambiguity according to the positivity criteria established by the World Anti-Doping Agency (WADA). Moreover, an isocratic method was developed to separate ephedrine from its isomer pseudoephedrine and cathine from phenylpropanolamine in a single run, what allowed their direct quantification in urine.
Keywords
Fast Analysis, Ultra-High-Pressure Liquid Chromatography, Quadrupole Time-of-Flight, Confirmatory Analysis, Doping Agents, Matrix Effect, Ephedrines, Quantitative Analysis, Laser-Induced Fluorescence, Lc-Ms/Ms Method, High-Throughput, Quantification, Electrophoresis, Validation, Ephedrines, Corticosteroids, Identification, Quantitation
Pubmed
Web of science
Create date
06/07/2010 9:52
Last modification date
20/08/2019 13:14
Usage data