Expression of fibroblast growth factors in ultraviolet radiation-induced corneal tumors and corneal tumor cell lines from Monodelphis domestica

Details

Serval ID
serval:BIB_2F8AC073F57B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Expression of fibroblast growth factors in ultraviolet radiation-induced corneal tumors and corneal tumor cell lines from Monodelphis domestica
Journal
Molecular Carcinogenesis
Author(s)
Sabourin  C. L., Kusewitt  D. F., Applegate  L. A., Budge  C. L., Ley  R. D.
ISSN
0899-1987 (Print)
Publication state
Published
Issued date
1993
Volume
7
Number
3
Pages
197-205
Notes
DA - 19930615
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (DNA, Neoplasm)
RN - 0 (RNA, Messenger)
RN - 0 (RNA, Neoplasm)
RN - 0 (Transforming Growth Factor beta)
RN - 103107-01-3 (Fibroblast Growth Factor 2)
RN - 104781-85-3 (Fibroblast Growth Factor 1)
SB - IM
Abstract
Chronic exposure of the gray, short-tailed opossum, Monodelphis domestica, to ultraviolet radiation (UVR) induces highly vascularized mesenchymal tumors of the cornea. Cell lines derived from these UVR-induced corneal tumors and the corneal tumors themselves were examined for the presence of mRNA coding for basic and acidic fibroblast growth factors (FGF), transforming growth factors-beta and -alpha (TGF-beta and TGF-alpha), epidermal growth factor (EGF), and tumor necrosis factor-alpha (TNF-alpha). Basic FGF was expressed in the cell lines derived from corneal tumors and in the corneal tumors. Expression of basic FGF was high in one corneal tumor. Transcripts for acidic FGF were detected only in the corneal tumor cell lines, not in primary tumors. TGF-beta expression was detected in the corneal tumors and tumor-derived cell lines. TGF-alpha, EGF, and TNF-alpha transcripts were not detectable in any opossum material; however, homologous gene sequences for TGF-alpha and EGF were detected on Southern blots of opossum genomic DNA. Southern blot analysis revealed no evidence of amplification or rearrangement of the genes for basic FGF or acidic FGF in the UVR-induced corneal tumor that expressed high levels of basic FGF. Opossum basic FGF, which stimulated the proliferation of fetal bovine heart endothelial cells, was purified by heparin affinity chromatography from a UVR-induced corneal tumor and a corneal tumor cell line. Immunoblotting of opossum basic FGF from a corneal tumor cell line using antiserum to bovine basic FGF showed two prominent immunoreactive bands of 17.5 and 18.5 kDa. Expression of basic FGF and acidic FGF may play a role in the development and progression of UVR-induced corneal tumors in M. domestica
Keywords
analysis/Animals/Cell Line/Cells/Cornea/Corneal Diseases/cytology/Dna/DNA,Neoplasm/Eye Neoplasms/Fibroblast Growth Factor 1/Fibroblast Growth Factor 2/Gene Expression/Gene Rearrangement/genetics/metabolism/Neoplasms,Radiation-Induced/Opossums/RNA,Messenger/RNA,Neoplasm/Transforming Growth Factor beta/Ultraviolet Rays
Pubmed
Web of science
Create date
18/02/2008 17:33
Last modification date
20/08/2019 13:14
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