Protection from radiation-induced colitis requires MHC class II antigen expression by cells of hemopoietic origin.

Details

Serval ID
serval:BIB_2F3A651863E8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Protection from radiation-induced colitis requires MHC class II antigen expression by cells of hemopoietic origin.
Journal
Journal of immunology
Author(s)
Marguerat S., MacDonald H.R., Kraehenbuhl J.P., van Meerwijk J.P.
ISSN
0022-1767
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
163
Number
7
Pages
4033-4040
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Ulcerative colitis, an inflammatory bowel disease, is believed to result from a breakdown of dominant tolerance mechanisms that normally control intestinal immunity. Although CD4+ T lymphocyte subpopulations and expression of MHC class II molecules have been shown to play a role in the pathogenesis of the disease, the nature of the responsible mechanisms remains unclear. In this paper we describe a novel mouse model for inflammatory bowel disease, radiation-induced colitis, that occurs with complete penetrance 6-8 wk postinduction. A combination of high dose gamma-irradiation and lack of MHC class II expression on cells of hemopoietic origin results in development of colitis in C57BL/6 mice. Because of its versatility (due to susceptibility of mice of the widely genetically manipulated C57BL/6 background), high reproducibility, and 100% penetrance, radiation-induced colitis will be a useful mouse model for colitis and a significant tool to study dominant immunological tolerance mechanisms. Moreover, our data imply that tolerization to enteric Ags requires MHC class II mediated presentation by APC of hemopoietic origin.
Keywords
Animals, Bone Marrow Cells/immunology, Bone Marrow Cells/metabolism, CD4-Positive T-Lymphocytes/immunology, CD4-Positive T-Lymphocytes/radiation effects, CD8-Positive T-Lymphocytes/immunology, CD8-Positive T-Lymphocytes/radiation effects, Colitis/genetics, Colitis/immunology, Dendritic Cells/immunology, Dendritic Cells/metabolism, Hematopoietic Stem Cells/immunology, Hematopoietic Stem Cells/metabolism, Histocompatibility Antigens Class I/genetics, Histocompatibility Antigens Class I/radiation effects, Histocompatibility Antigens Class II/biosynthesis, Histocompatibility Antigens Class II/genetics, Interferon-gamma/biosynthesis, Intestinal Mucosa/immunology, Intestinal Mucosa/metabolism, Lymphocyte Subsets/immunology, Lymphocyte Subsets/metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, Radiation Chimera/genetics, Radiation Chimera/immunology
Pubmed
Web of science
Create date
25/01/2008 16:06
Last modification date
20/08/2019 14:13
Usage data