Article: article from journal or magazin.
In vitro immune recognition of synthetic peptides from the Plasmodium falciparum CS protein by individuals naturally exposed to different sporozoite challenge
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Jul
The impact of duration and intensity of sporozoite challenge on the in vitro cell immune response to synthetic peptides of the circumsporozoite (CS) protein of Plasmodium falciparum was investigated in residents of a malaria endemic area in Burkina Faso (West Africa). Lymphocyte proliferation and interferon-gamma (IFN-gamma) production were used to assess immune recognition of synthetic peptides corresponding to the polymorphic Th2R and Th3R regions, to the conserved CS.T3 sequence and to NANP and degenerate NVDP repeats. Immune responses were measured in adults and children from a village where they received more than 100 sporozoite inoculations per year and in adults living in a town, exposed to a 10-100 times lower challenge. A lifetime intense exposure apparently increased the ability to proliferate in response to most peptides in the rural adults, who all produced antibodies to NANP repeats. Surprisingly, cell cultures from these subjects seldom contained appreciable levels of IFN-gamma. In the urban adults, possibly due to the moderate challenge they are exposed to, significant differences in the proliferative potentials of the peptides could be detected. The highest stimulation indices were obtained with the genetically unrestricted CS.T3 peptide. Remarkably, proliferative responses to Th2R and Th3R appeared to be correlated with the humoral response to the CS protein, indicating a T helper significance of the epitopes. The differing proliferative potential of the polymorphic epitopes in the urban adults suggests that polymorphism might delay the development of immune responsiveness under conditions of sporadic transmission. The children from the highly malarious village displayed the lowest proliferative scores, accompanied by a high prevalence of antibodies to NANP repeats. On the basis of these findings, the hypothesis is proposed that a pure B cell reactivity to NANP repeats could ontogenetically precede the mounting of a conventional T-B cooperative immune response.
Adult Aged Amino Acid Sequence Animals Antibodies, Protozoan/immunology Antigen-Antibody Reactions/*immunology Antigens, Protozoan/*immunology Apicomplexa/immunology Burkina Faso/epidemiology Child Child, Preschool Female Humans Immunity, Cellular Interferon Type II/metabolism Leukocytes, Mononuclear/immunology/parasitology Lymphocyte Activation/immunology Malaria, Falciparum/epidemiology/*immunology Male Middle Aged Molecular Sequence Data Oligopeptides/chemical synthesis/*immunology Plasmodium falciparum/*immunology Protozoan Proteins/chemical synthesis/immunology
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