Effects of colostrum feeding and glucocorticoid administration on insulin-dependent glucose metabolism in neonatal calves

Details

Serval ID
serval:BIB_2E681EF9DAE7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effects of colostrum feeding and glucocorticoid administration on insulin-dependent glucose metabolism in neonatal calves
Journal
Domestic Animal Endocrinology
Author(s)
Scheuer  B. H., Zbinden  Y., Schneiter  P., Tappy  L., Blum  J. W., Hammon  H. M.
ISSN
0739-7240 (Print)
Publication state
Published
Issued date
10/2006
Volume
31
Number
3
Pages
227-45
Language
english
Notes
Journal Article Randomized Controlled Trial Research Support, Non-U.S. Gov't --- Old month value: Oct
Abstract
Colostrum feeding and glucocorticoid administration affect glucose metabolism and insulin release in calves. We have tested the hypothesis that dexamethasone as well as colostrum feeding influence insulin-dependent glucose metabolism in neonatal calves using the euglycemic-hyperinsulinemic clamp technique. Newborn calves were fed either colostrum or a milk-based formula (n=14 per group) and in each feeding group, half of the calves were treated with dexamethasone (30 microg/[kg body weight per day]). Preprandial blood samples were taken on days 1, 2, and 4. On day 5, insulin was infused for 3h and plasma glucose concentrations were kept at 5 mmol/L+/-10%. Clamps were combined with [(13)C]-bicarbonate and [6,6-(2)H]-glucose infusions for 5.5h (i.e., from -150 to 180 min, relative to insulin infusion) to determine glucose turnover, glucose appearance rate (Ra), endogenous glucose production (eGP), and gluconeogenesis before and at the end of the clamp. After the clamp liver biopsies were taken to measure mRNA levels of phosphoenolpyruvate carboxykinase (PEPCK) and pyruvate carboxylase (PC). Dexamethasone increased plasma glucose, insulin, and glucagon concentrations in the pre-clamp period thus necessitating a reduction in the rate of glucose infusion to maintain euglycemia during the clamp. Glucose turnover and Ra increased during the clamp and were lower at the end of the clamp in dexamethasone-treated calves. Dexamethasone treatment did not affect basal gluconeogenesis or eGP. At the end of the clamp, dexamethasone reduced eGP and PC mRNA levels, whereas mitochondrial PEPCK mRNA levels increased. In conclusion, insulin increased glucose turnover and dexamethasone impaired insulin-dependent glucose metabolism, and this was independent of different feeding.
Keywords
Animals Animals, Newborn Blood Glucose/*metabolism Body Weight/drug effects Cattle/blood/*metabolism Colostrum/*metabolism Dexamethasone/*pharmacology Eating/drug effects Fatty Acids, Nonesterified/blood Glucagon/blood Glucocorticoids/*pharmacology Glucose Clamp Technique/veterinary Insulin/blood/*metabolism Lactic Acid/blood Liver/enzymology Male Phosphoenolpyruvate Carboxykinase (ATP)/biosynthesis/genetics Pyruvate Carboxylase/biosynthesis/genetics RNA, Messenger/metabolism Urea/blood
Pubmed
Web of science
Create date
24/01/2008 13:36
Last modification date
20/08/2019 13:12
Usage data