Brain energy metabolism measured by (13) C magnetic resonance spectroscopy in vivo upon infusion of [3-(13) C]lactate.

Details

Serval ID
serval:BIB_2E42476B81C1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Brain energy metabolism measured by (13) C magnetic resonance spectroscopy in vivo upon infusion of [3-(13) C]lactate.
Journal
Journal of Neuroscience Research
Author(s)
Duarte J.M., Girault F.M., Gruetter R.
ISSN
1097-4547 (Electronic)
ISSN-L
0360-4012
Publication state
Published
Issued date
07/2015
Peer-reviewed
Oui
Volume
93
Number
7
Pages
1009-1018
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The brain uses lactate produced by glycolysis as an energy source. How lactate originated from the blood stream is used to fuel brain metabolism is not clear. The current study measures brain metabolic fluxes and estimates the amount of pyruvate that becomes labeled in glial and neuronal compartments upon infusion of [3-(13) C]lactate. For that, labeling incorporation into carbons of glutamate and glutamine was measured by (13) C magnetic resonance spectroscopy at 14.1 T and analyzed with a two-compartment model of brain metabolism to estimate rates of mitochondrial oxidation, glial pyruvate carboxylation, and the glutamate-glutamine cycle as well as pyruvate fractional enrichments. Extracerebral lactate at supraphysiological levels contributes at least two-fold more to replenish the neuronal than the glial pyruvate pools. The rates of mitochondrial oxidation in neurons and glia, pyruvate carboxylase, and glutamate-glutamine cycles were similar to those estimated by administration of (13) C-enriched glucose, the main fuel of brain energy metabolism. These results are in agreement with primary utilization of exogenous lactate in neurons rather than astrocytes. © 2014 Wiley Periodicals, Inc.
Pubmed
Web of science
Create date
23/12/2014 9:03
Last modification date
20/08/2019 13:12
Usage data