Expression of somatostatin receptor types 2, 3 and 5 in biopsies and surgical specimens of human lung tumours. Correlation with preoperative octreotide scintigraphy.

Details

Serval ID
serval:BIB_2DF0EAAB9852
Type
Article: article from journal or magazin.
Collection
Publications
Title
Expression of somatostatin receptor types 2, 3 and 5 in biopsies and surgical specimens of human lung tumours. Correlation with preoperative octreotide scintigraphy.
Journal
Virchows Archiv
Author(s)
Papotti M., Croce S., Bellò M., Bongiovanni M., Allìa E., Schindler M., Bussolati G.
ISSN
0945-6317 (Print)
ISSN-L
0945-6317
Publication state
Published
Issued date
2001
Peer-reviewed
Oui
Volume
439
Number
6
Pages
787-797
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
The increasingly popular use of somatostatin analogs in clinical practice for both diagnostic and therapeutic purposes prompted extensive investigations on somatostatin receptor (sst) expression in human tumors by autoradiography, nucleic acid analysis and, recently, immunohistochemistry (IHC). The currently employed radiotracer for scintigraphy (Octreoscan) is octreotide, a somatostatin analog having a high affinity for sst types 2, 3, and 5. In this study on 25 patients, we compared sst 2, 3, and 5 expression in surgical and biopsy specimens of lung tumors, as revealed by immunohistochemical and reverse transcriptase polymerase chain reaction (RT-PCR), with the octreoscan outcome (which was positive in 20/25 cases). By IHC, the tumors mainly expressed sst2 (17/25, 68%) at the cell membrane level, while sst 3 and 5 were detected in a fraction of cases (24% and 20%, respectively). Comparing RT-PCR and IHC data, a correlation was found in 83.3% of cases, while octreoscan findings and sst expression were correlated in 22/25 cases (88%). In addition, cytological and biopsy specimens expressed the same sst type found in the corresponding surgical sample, thus indicating that a cell membrane sst immunoreactivity in a biopsy reliably predicts the tumor-receptor profile before its resection. Finally, sst expression was not restricted to neuroendocrine lung tumors, but was also a feature of some non-neuroendocrine carcinomas, although to a lesser extent. The occasional expression of sst subtypes in intratumoral lymphocytes, endothelia and necrotic areas is an additional feature to be considered in the interpretation of Octreoscan findings, since the in vivo procedure does not allow to define the sst cellular distribution. IHC can therefore be usefully coupled to radionuclear investigations to better characterize the sst cellular location and subtype in lung tumors.
Keywords
Adenocarcinoma/metabolism, Adenocarcinoma/pathology, Adult, Aged, Biopsy, DNA Primers/chemistry, Female, Humans, Immunoenzyme Techniques, Lung Neoplasms/metabolism, Lung Neoplasms/pathology, Male, Middle Aged, Octreotide/diagnostic use, Preoperative Care, RNA, Neoplasm/analysis, Receptors, Somatostatin/biosynthesis, Receptors, Somatostatin/genetics, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Web of science
Create date
05/02/2015 15:07
Last modification date
20/08/2019 13:12
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