Bacteriophages Improve Outcome in Experimental Staphylococcus Aureus Ventilator Associated Pneumonia.

Détails

ID Serval
serval:BIB_2CF5B9B31B06
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Bacteriophages Improve Outcome in Experimental Staphylococcus Aureus Ventilator Associated Pneumonia.
Périodique
American journal of respiratory and critical care medicine
Auteur(s)
Prazak J., Iten M., Cameron D.R., Save J., Grandgirard D., Resch G., Goepfert C., Leib S.L., Takala J., Jakob S.M., Que Y.A., Haenggi M.
ISSN
1535-4970 (Electronic)
ISSN-L
1073-449X
Statut éditorial
In Press
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Résumé
Infections caused by multidrug resistant bacteria are a major clinical challenge. Phage therapy is a promising alternative antibacterial strategy.
To evaluate the efficacy of intravenous phage therapy for the treatment of ventilator associated pneumonia due to methicillin-resistant Staphylococcus aureus in rats.
A randomized blinded controlled experimental study compared intravenous teicoplanin (3mg/kg, n=12), a cocktail of four phages (2-3 x 10^9 plaque forming units/ml of 2003, 2002, 3A and K, n=12) and combination of both (n=11), given two, 12 and 24 hours after induction of pneumonia, then once daily for four days. The primary outcome was survival at day four. Secondary outcomes were bacterial and phage densities in lungs and spleen, histopathological scoring of infection within the lungs and inflammatory biomarkers in blood.
Treatment with either phages or teicoplanin increased survival from 0% to 58% and 50% respectively (p<0.005). Combination of phage with antibiotics did not further improve outcome (45% survival). Animal survival correlated with reduced bacterial burden in the lung (1.2 x 10^6 CFU/g of tissue for survivors versus 1.2 x 10^9 CFU/g for non-surviving animals, p<0.0001), as well as improved histopathological outcomes. Phage multiplication within the lung occurred during treatment. IL-1β increased for all treatment groups over the course of therapy.
Phage therapy was as effective as teicoplanin in improving survival and decreasing bacterial load within the lungs of rats infected with methicillin-resistant S. aureus. Combining antibiotics with phage therapy did not further improve outcomes. Key Words: bacteriophage; antibiotic resistance, microbial; pneumonia, ventilator associated.
Mots-clé
bacteriophage, antibiotic resistance, microbial, pneumonia, ventilator associated
Pubmed
Création de la notice
18/07/2019 16:22
Dernière modification de la notice
21/08/2019 5:34
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