Design of in vitro Transcribed mRNA Vectors for Research and Therapy.

Détails

ID Serval
serval:BIB_2CCF1F79C61C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Design of in vitro Transcribed mRNA Vectors for Research and Therapy.
Périodique
Chimia
Auteur(s)
Tusup M., French L.E., De Matos M., Gatfield D., Kundig T., Pascolo S.
ISSN
0009-4293 (Print)
ISSN-L
0009-4293
Statut éditorial
Publié
Date de publication
29/05/2019
Peer-reviewed
Oui
Volume
73
Numéro
6
Pages
391-394
Langue
anglais
Résumé
The use of in vitro transcribed messenger RNA (ivt mRNA) for vaccination, gene therapy and cell reprograming has become increasingly popular in research and medicine. This method can be used in vitro (transfected in cells) or administered naked or formulated (lipoplexes, polyplexes, and lipopolyplexes that deliver the RNA to specific organs, such as immune structures, the lung or liver) and is designed to be an immunostimulatory or immunosilent agent. This vector contains several functional regions (Cap, 5' untranslated region, open reading frame, 3' untranslated region and poly-A tail) that can all be optimised to generate a highly efficacious ivt mRNA. In this study, we review these aspects and report on the effect of the ivt mRNA purification method on the functionality of this synthetic transient genetic vector.
Mots-clé
3' Untranslated Regions, Genetic Therapy, RNA, Messenger/genetics, Research
Pubmed
Web of science
Création de la notice
27/05/2019 10:24
Dernière modification de la notice
20/08/2019 14:11
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