Cuticular Defects in Oryza sativa ATP-binding Cassette Transporter G31 Mutant Plants Cause Dwarfism, Elevated Defense Responses and Pathogen Resistance.

Détails

ID Serval
serval:BIB_2C852EFA401D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cuticular Defects in Oryza sativa ATP-binding Cassette Transporter G31 Mutant Plants Cause Dwarfism, Elevated Defense Responses and Pathogen Resistance.
Périodique
Plant & cell physiology
Auteur(s)
Garroum I., Bidzinski P., Daraspe J., Mucciolo A., Humbel B.M., Morel J.B., Nawrath C.
ISSN
1471-9053 (Electronic)
ISSN-L
0032-0781
Statut éditorial
Publié
Date de publication
04/2016
Peer-reviewed
Oui
Volume
57
Numéro
6
Pages
1179-1188
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The cuticle covers the surface of the polysaccharide cell wall of leaf epidermal cells and forms an essential diffusion barrier between plant and environment. Homologs of the ATP-binding cassette (ABC) transporter AtABCG32/HvABCG31 clade are necessary for the formation of a functional cuticle in both monocots and dicots. Here we characterize the osabcg31 knockout mutant and hairpin RNA interference (RNAi)-down-regulated OsABCG31 plant lines having reduced plant growth and a permeable cuticle. The reduced content of cutin in leaves and structural alterations in the cuticle and at the cuticle-cell wall interface in plants compromised in OsABCG31 expression explain the cuticle permeability. Effects of modifications of the cuticle on plant-microbe interactions were evaluated. The cuticular alterations in OsABCG31-compromised plants did not cause deficiencies in germination of the spores or the formation of appressoria of Magnaporthe oryzae on the leaf surface, but a strong reduction of infection structures inside the plant. Genes involved in pathogen resistance were constitutively up-regulated in OsABCG31-compromised plants, thus being a possible cause of the resistance to M. oryzae and the dwarf growth phenotype. The findings show that in rice an abnormal cuticle formation may affect the signaling of plant growth and defense.

Mots-clé
ATP-Binding Cassette Transporters/genetics, ATP-Binding Cassette Transporters/metabolism, Disease Resistance/genetics, Gene Expression Regulation, Plant, Magnaporthe/physiology, Membrane Lipids/metabolism, Mutation/genetics, Oryza/anatomy & histology, Oryza/genetics, Oryza/growth & development, Oryza/immunology, Phenotype, Plant Diseases/microbiology, Plant Epidermis/genetics, Plant Epidermis/ultrastructure, Plant Proteins/genetics, Plant Proteins/metabolism
Pubmed
Open Access
Oui
Création de la notice
17/05/2016 18:07
Dernière modification de la notice
20/08/2019 13:11
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