Drainage of fluorescent liposomes from the vitreous to cervical lymph nodes via conjunctival lymphatics.

Détails

ID Serval
serval:BIB_2BFECDDFEF0F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Drainage of fluorescent liposomes from the vitreous to cervical lymph nodes via conjunctival lymphatics.
Périodique
Ophthalmic Research
Auteur(s)
Camelo S., Lajavardi L., Bochot A., Goldenberg B., Naud M.C., Fattal E., Behar-Cohen F., de Kozak Y.
ISSN
1423-0259 (Electronic)
ISSN-L
0030-3747
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
40
Numéro
3-4
Pages
145-150
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
The use of liposomes as carriers for the delivery of biologically active molecules into the eye is of major interest. Indeed, encapsulation of biologically active molecules in liposomes may increase their bioavailability and may induce a sustained release, thus avoiding repeated intraocular injections and reducing side effects. We describe here the fate of rhodamine-conjugated liposomes (Rh-Lip) injected into the vitreous of normal Lewis rats. Twenty-four hours after intravitreal injection fluorescent liposomes were detected in the vitreous, the inner layer of the retina and to a lesser extent in the anterior segment of the eye. In addition, numerous Rh-Lip were also observed in the episclera and conjunctival stroma, in conjunctival lymphatic vessels and cervical lymph nodes (LN) draining the conjunctiva and the eye. In the LN, Rh-Lip were taken up by resident macrophages adjacent to CD4+ and CD8+ T cells. Thus, intravitreal injection of anti-inflammatory drugs loaded in liposomes could modulate the ocular immune microenvironment. In addition the passage of drugs into the cervical LN could alter the immune status of these LN and contribute to the regulation of intraocular inflammation. Our results suggest that this phenomenon should be taken into account to design new therapies based on intraocular drug administration.
Mots-clé
Animals, Conjunctiva/metabolism, Fluorescent Dyes/metabolism, Immunohistochemistry, Injections, Liposomes, Lymph Nodes/metabolism, Lymphatic System/physiology, Male, Microscopy, Confocal, Neck, Rats, Rats, Inbred Lew, Retina/metabolism, Rhodamines/metabolism, Vitreous Body/metabolism
Pubmed
Web of science
Création de la notice
27/08/2013 11:09
Dernière modification de la notice
20/03/2018 13:20
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