Random amplification of polymorphic bacterial DNA: evaluation of 11 oligonucleotides and application to food contaminated with Listeria monocytogenes.

Détails

ID Serval
serval:BIB_2BF9AC1F5402
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Random amplification of polymorphic bacterial DNA: evaluation of 11 oligonucleotides and application to food contaminated with Listeria monocytogenes.
Périodique
Journal of Applied Bacteriology
Auteur(s)
Niederhauser C., Höfelein C., Allmann M., Burkhalter P., Lüthy J., Candrian U.
ISSN
0021-8847 (Print)
ISSN-L
0021-8847
Statut éditorial
Publié
Date de publication
1994
Peer-reviewed
Oui
Volume
77
Numéro
5
Pages
574-582
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article Publication Status: ppublish
Résumé
The polymerase chain reaction was used to obtain randomly-amplified polymorphic DNA (RAPD) profiles from Listeria spp. and enterobacteria. Eleven different oligonucleotides were evaluated. Only one, HR4 (19mer), generated reproducible and specific profiles for Listeria spp., while results for enterobacteria were controversial. A total of 57 different Listeria strains were subjected to the RAPD analysis and 27 different profiles were recognized. RAPD typing allowed strains of the same serotype to be distinguished but the same profile was obtained from different serotypes of L. monocytogenes in three cases and in one case two different serotypes of L. innocua yielded the same profile. RAPD-typing with HR4 allowed L. monocytogenes contamination in several food outlets to be traced back to a food processing plant. In additional experiments, the general utility of this RAPD system in typing Yersinia enterocolitica, verotoxigenic Escherichia coli and Salmonella enteritidis was evaluated.
Mots-clé
Bacterial Typing Techniques, Base Sequence, DNA, Bacterial/genetics, DNA, Bacterial/isolation & purification, Enterobacteriaceae/classification, Enterobacteriaceae/genetics, Evaluation Studies as Topic, Food Contamination, Food Handling, Food Microbiology, Listeria monocytogenes/classification, Listeria monocytogenes/genetics, Meat Products/microbiology, Molecular Sequence Data, Oligonucleotides, Polymerase Chain Reaction/methods, Polymorphism, Genetic
Pubmed
Web of science
Création de la notice
09/11/2014 16:44
Dernière modification de la notice
03/03/2018 15:26
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