Antegrade versus retrograde isolated lung perfusion: doxorubicin uptake and distribution in a sarcoma model.

Détails

ID Serval
serval:BIB_2BEC657CCDDF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Antegrade versus retrograde isolated lung perfusion: doxorubicin uptake and distribution in a sarcoma model.
Périodique
Annals of Thoracic Surgery
Auteur(s)
Krueger T., Kuemmerle A., Andrejevic-Blant S., Yan H., Pan Y., Ballini J.P., Klepetko W., Decosterd L.A., Stupp R., Ris H.B.
ISSN
1552-6259[electronic]
Statut éditorial
Publié
Date de publication
2006
Volume
82
Numéro
6
Pages
2024-2030
Langue
anglais
Résumé
BACKGROUND: Antegrade and retrograde doxorubicin-based isolated lung perfusions were compared in rodents bearing a sarcomatous tumor in the perfused lung. Inasmuch as these tumors derive their vascularization from the bronchial artery system, we hypothesized that retrograde isolated lung perfusion through the pulmonary vein might result in an improved tumor drug uptake. METHODS: Single-pass antegrade (n = 9) and retrograde (n = 9) isolated left lung perfusions were performed with 100 microg of doxorubicin in Fischer rats 10 days after subpleural tumor cell injection. The perfusion, washout, and recirculation times were 20, 10, and 60 minutes, respectively, followed by harvesting of the lung. The doxorubicin concentration and compartmental distribution in the tumor and in normal parenchyma of each perfused lung were measured by high-pressure liquid chromatography (6 animals of each group) and fluorescence microscopy (3 animals of each group). RESULTS: Doxorubicin concentration and pattern of doxorubicin-based fluorescence signaling were comparable for both perfusion techniques in normal lung tissue. Antegrade and retrograde isolated lung perfusion resulted in similar tumor drug uptake, which was lower than in normal lung parenchyma, and in weak and sporadic fluorescence signaling emerging from the tumor periphery and from blood vessels situated within the tumor tissue. CONCLUSIONS: Retrograde isolated lung perfusion did not confer a better doxorubicin uptake in the tumor as compared with antegrade lung perfusion despite the fact that the tumor vascularization in this model is based on the bronchial artery circulation.
Mots-clé
Animals, Antibiotics, Antineoplastic, Cell Line, Tumor, Chemotherapy, Cancer, Regional Perfusion, Doxorubicin, Lung Neoplasms, Male, Rats, Rats, Inbred F344, Sarcoma, Xenograft Model Antitumor Assays
Pubmed
Web of science
Création de la notice
28/01/2008 9:39
Dernière modification de la notice
03/03/2018 15:26
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