HCF-2 inhibits cell proliferation and activates differentiation-gene expression programs.

Détails

ID Serval
serval:BIB_2BB8B41DAB1A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
HCF-2 inhibits cell proliferation and activates differentiation-gene expression programs.
Périodique
Nucleic acids research
Auteur(s)
Gudkova D., Dergai O., Praz V., Herr W.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Statut éditorial
Publié
Date de publication
20/06/2019
Peer-reviewed
Oui
Volume
47
Numéro
11
Pages
5792-5808
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
HCF-2 is a member of the host-cell-factor protein family, which arose in early vertebrate evolution as a result of gene duplication. Whereas its paralog, HCF-1, is known to act as a versatile chromatin-associated protein required for cell proliferation and differentiation, much less is known about HCF-2. Here, we show that HCF-2 is broadly present in human and mouse cells, and possesses activities distinct from HCF-1. Unlike HCF-1, which is excluded from nucleoli, HCF-2 is nucleolar-an activity conferred by one and a half C-terminal Fibronectin type 3 repeats and inhibited by the HCF-1 nuclear localization signal. Elevated HCF-2 synthesis in HEK-293 cells results in phenotypes reminiscent of HCF-1-depleted cells, including inhibition of cell proliferation and mitotic defects. Furthermore, increased HCF-2 levels in HEK-293 cells lead to inhibition of cell proliferation and metabolism gene-expression programs with parallel activation of differentiation and morphogenesis gene-expression programs. Thus, the HCF ancestor appears to have evolved into a small two-member protein family possessing contrasting nuclear versus nucleolar localization, and cell proliferation and differentiation functions.
Pubmed
Open Access
Oui
Création de la notice
27/05/2019 18:07
Dernière modification de la notice
21/08/2019 6:37
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