Microfluidics for rapid cytokeratin immunohistochemical staining in frozen sections.

Détails

ID Serval
serval:BIB_2B9CC70589A8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Microfluidics for rapid cytokeratin immunohistochemical staining in frozen sections.
Périodique
Laboratory investigation; a journal of technical methods and pathology
Auteur(s)
Brajkovic S., Dupouy D.G., de Leval L., Gijs M.A.
ISSN
1530-0307 (Electronic)
ISSN-L
0023-6837
Statut éditorial
Publié
Date de publication
08/2017
Peer-reviewed
Oui
Volume
97
Numéro
8
Pages
983-991
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Frozen sections (FS) of tumor samples represent a cornerstone of pathological intraoperative consultation and have an important role in the microscopic analysis of specimens during surgery. So far, immunohistochemical (IHC) stainings on FS have been demonstrated for a few markers using manual methods. Microfluidic technologies have proven to bring substantial improvement in many fields of diagnostics, though only a few microfluidic devices have been designed to improve the performance of IHC assays. In this work, we show optimization of a complete pan-cytokeratin chromogenic immunostaining protocol on FS using a microfluidic tissue processor into a protocol taking <12 min. Our results showed specificity and low levels of background. The dimensions of the microfluidic prototype device are compatible with the space constraints of an intraoperative pathology laboratory. We therefore anticipate that the adoption of microfluidic technologies in the field of surgical pathology can significantly improve the way FSs influence surgical procedures.

Mots-clé
Breast/diagnostic imaging, Coloring Agents/chemistry, Equipment Design, Female, Humans, Immunohistochemistry/instrumentation, Immunohistochemistry/methods, Keratins/analysis, Keratins/chemistry, Keratins/metabolism, Male, Microfluidic Analytical Techniques/instrumentation, Microfluidic Analytical Techniques/methods, Neoplasms/diagnostic imaging, Prostate/diagnostic imaging, Ureter/diagnostic imaging
Pubmed
Web of science
Open Access
Oui
Création de la notice
31/05/2017 13:08
Dernière modification de la notice
08/05/2019 16:21
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