Nerve-independence of limb regeneration in larval Xenopus laevis is correlated to the level of fgf-2 mRNA expression in limb tissues.

Détails

ID Serval
serval:BIB_2B9A99EF2202
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Nerve-independence of limb regeneration in larval Xenopus laevis is correlated to the level of fgf-2 mRNA expression in limb tissues.
Périodique
Developmental biology
Auteur(s)
Cannata S.M., Bagni C., Bernardini S., Christen B., Filoni S.
ISSN
0012-1606 (Print)
ISSN-L
0012-1606
Statut éditorial
Publié
Date de publication
15/03/2001
Volume
231
Numéro
2
Pages
436-446
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
In both larval and adult urodele amphibians, limb blastema formation requires the presence of an adequate nerve supply. In previous research, we demonstrated that the hindlimb of early Xenopus laevis larvae formed a regeneration blastema even when denervated, while the denervated limb of late larvae did not. We hypothesized that the nerve-independence was due to the autonomous synthesis of a mitogenic neurotrophic-like factor by undifferentiated limb bud cells. In this paper, we demonstrate that fgf-2 mRNA is present in larval limb tissues and that its level is correlated to the extent of mesenchymal cells populating the limb: in early limbs, fgf-2 mRNA is present at high levels all over the limb, while, in late limbs, the fgf-2 expression is low and detectable only in the distal autopodium. After denervation, fgf-2 mRNA synthesis increases in amputated early limbs but not in amputated late limbs. The implantation of anti-FGF-2 beads into amputated early limbs hardly lowers the mitotic activity of blastema cells. However, FGF-2 beads implanted into the blastema of late limbs prevent the denervation-induced inhibition of mitosis and oppose blastema regression. Our data indicate that FGF-2 is a good candidate for the endogenous mitogenic factor responsible for blastema formation and growth in amputated and denervated early limbs. However, in amputated late limbs, the very limited fgf-2 expression is not sufficient to promote blastema formation in the absence of nerves.

Mots-clé
Animals, Cell Division, Extremities/innervation, Extremities/physiology, Fibroblast Growth Factor 2/biosynthesis, Fibroblast Growth Factor 2/metabolism, In Situ Hybridization, Larva/physiology, Mesoderm/metabolism, Polyribosomes/metabolism, RNA, Messenger/metabolism, Regeneration, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, Xenopus laevis
Pubmed
Open Access
Oui
Création de la notice
06/03/2017 18:23
Dernière modification de la notice
08/05/2019 16:21
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